Effects of synthetic pyrethroids and methidation on activities of some digestive enzymes in carp (Cyprinus carpio L.)

L. M. Simon, K. László, M. Kotormán, A. Vértesi, K. Bagi, J. Nemcsók

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effects of pyrethroid pesticides (deltamethrin, permethrin and cypermethrin) and an organophosphate ester (methidation) on the activities of carp trypsin, α-chymotrypsin, carboxypeptidase A and lipase were studied. The enzymes were isolated from the gastrointestinal tract and the effects of the pesticides were investigated during incubation for 5 min. The activity of trypsin was influenced only slightly by the presence of deltamethrin and methidation, whereas permethrin and cypermethrin caused significant inhibition. The pyrethroid pesticides at lower concentrations resulted in a slight activation of α-chymotrypsin. Methidation inhibited the α- chymotrypsin activity by about 20%. These pesticides modified the lipase activity to a lesser extent; the highest inhibition was measured with cypermethrin. The carboxypeptidase A activity was inhibited by both pyrethroid pesticides and methidation. The results suggest that these pesticides might interact with the active conformation of the studied hydrolytic enzymes, resulting in changes in their activities.

Original languageEnglish
Pages (from-to)819-828
Number of pages10
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume34
Issue number5
Publication statusPublished - 1999

Fingerprint

Pyrethrins
cypermethrin
Carps
pyrethroid
digestive enzymes
pyrethrins
Pesticides
Cyprinus carpio
carp
deltamethrin
permethrin
pesticides
Enzymes
pesticide
enzyme
chymotrypsin
carboxypeptidase A
Carboxypeptidases A
Permethrin
organophosphate

Keywords

  • α- chymotrypsin
  • Carboxypeptidase A
  • Carp
  • Cypermethrin
  • Deltamethrin
  • Lipase activity
  • Methidation
  • Permethrin
  • Trypsin

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Waste Management and Disposal

Cite this

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abstract = "The effects of pyrethroid pesticides (deltamethrin, permethrin and cypermethrin) and an organophosphate ester (methidation) on the activities of carp trypsin, α-chymotrypsin, carboxypeptidase A and lipase were studied. The enzymes were isolated from the gastrointestinal tract and the effects of the pesticides were investigated during incubation for 5 min. The activity of trypsin was influenced only slightly by the presence of deltamethrin and methidation, whereas permethrin and cypermethrin caused significant inhibition. The pyrethroid pesticides at lower concentrations resulted in a slight activation of α-chymotrypsin. Methidation inhibited the α- chymotrypsin activity by about 20{\%}. These pesticides modified the lipase activity to a lesser extent; the highest inhibition was measured with cypermethrin. The carboxypeptidase A activity was inhibited by both pyrethroid pesticides and methidation. The results suggest that these pesticides might interact with the active conformation of the studied hydrolytic enzymes, resulting in changes in their activities.",
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T1 - Effects of synthetic pyrethroids and methidation on activities of some digestive enzymes in carp (Cyprinus carpio L.)

AU - Simon, L. M.

AU - László, K.

AU - Kotormán, M.

AU - Vértesi, A.

AU - Bagi, K.

AU - Nemcsók, J.

PY - 1999

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N2 - The effects of pyrethroid pesticides (deltamethrin, permethrin and cypermethrin) and an organophosphate ester (methidation) on the activities of carp trypsin, α-chymotrypsin, carboxypeptidase A and lipase were studied. The enzymes were isolated from the gastrointestinal tract and the effects of the pesticides were investigated during incubation for 5 min. The activity of trypsin was influenced only slightly by the presence of deltamethrin and methidation, whereas permethrin and cypermethrin caused significant inhibition. The pyrethroid pesticides at lower concentrations resulted in a slight activation of α-chymotrypsin. Methidation inhibited the α- chymotrypsin activity by about 20%. These pesticides modified the lipase activity to a lesser extent; the highest inhibition was measured with cypermethrin. The carboxypeptidase A activity was inhibited by both pyrethroid pesticides and methidation. The results suggest that these pesticides might interact with the active conformation of the studied hydrolytic enzymes, resulting in changes in their activities.

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KW - Trypsin

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