Effects of storage duration and temperature of human blood on red cell deformability and aggregation

Mehmet Uyuklu, Melike Cengiz, Pinar Ulker, Timea Hever, Julien Tripette, Philippe Connes, Norbert Nemeth, Herbert J. Meiselman, Oguz K. Baskurt

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42 Citations (Scopus)

Abstract

Blood samples used in hemorheological studies may be stored for a period of time, the effects of storage have yet to be fully explored. This study evaluated the effects of storage temperature (i.e., 4°C or 25°C) and duration on RBC deformability and aggregation for blood from healthy controls and from septic patients. Our results indicate that for normal blood, RBC deformability over 0.3-50 Pa is stable up to six hours regardless of storage temperature; at eight hours there were no significant differences in EI but SS 1/2 calculated via a Lineweaver-Burk method indicated impaired deformability. Storage temperature affected the stable period for RBC aggregation: the safe time was shorter at 25°C whereas at 4°C aggregation was stable up to 12 hours. Interestingly, blood samples from septic patients were less affected by storage. Blood can thus be stored at 25°C for up to six hours for deformability studies, but should be limited to four hours for RBC aggregation; storage at 4°C may prolong the storage period up to 12 hours for aggregation but not deformability measurements. Therefore, the time period between sampling and measurement should be as short as possible and reported together with results.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalClinical hemorheology and microcirculation
Volume41
Issue number4
DOIs
Publication statusPublished - Jul 31 2009

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Keywords

  • Aggregation
  • Blood storage
  • Deformability
  • Temperature

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Uyuklu, M., Cengiz, M., Ulker, P., Hever, T., Tripette, J., Connes, P., Nemeth, N., Meiselman, H. J., & Baskurt, O. K. (2009). Effects of storage duration and temperature of human blood on red cell deformability and aggregation. Clinical hemorheology and microcirculation, 41(4), 269-278. https://doi.org/10.3233/CH-2009-1178