Effects of sodium fluoride (NaF) on the cilia and microtubular system of Tetrahymena

P. Kovács, E. Pállinger, G. Csaba

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of the nucleophilic reagent NaF on the microtubular system of Tetrahymena was studied by using scanning electron microscopy (SEM), confocal microscopy, and flow cytometry. Treatments with 40 mM NaF significantly reduced the amount of α-tubulin while 80 mM treatment did not alter its quantity. One possible explanation for this α-tubulin overexpression is that the higher amount of α-tubulin enables this organism to carry out the appropriate function of the cytoskeleton under this undesirable influence of higher amounts of 80 nM NaF. However, the amount of acetylated tubulin increased in a dose-dependent manner. The cilia became fragile under the effect of 80 mM NaF. Confocal microscopy revealed that after 40 mM NaF treatment transversal microtubule bands (TMs) and longitudinal microtubule bands (LMs) as well as basal bodies (BBs) were extremely strong decorated with anti-acetylated tubulin antibody and TM-localization abnormalities were visible. In the 80 mM NaF-treated cells, the deep fiber of oral apparatus was very strongly labeled, while the TMs and LMs were less decorated with anti-acetylated tubulin antibody, and LM deformities were visible. It is supposed that post-translational tubulin modifications (e.g., acetylation) defend the microtubules against the NaF-induced injury. NaF is able to influence the activity of several enzymes and G-proteins, therefore is capable to alter the structure, metabolism, and the dynamics of microtubular system. The possible connection of signaling and cytoskeletal system in Tetrahymena is discussed.

Original languageEnglish
Pages (from-to)591-597
Number of pages7
JournalCell Biochemistry and Function
Volume26
Issue number5
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Tetrahymena
Sodium Fluoride
Cilia
Tubulin
Microtubules
Confocal microscopy
Confocal Microscopy
Basal Bodies
Acetylation
Flow cytometry
Post Translational Protein Processing
Cytoskeleton
GTP-Binding Proteins
Metabolism
Electron Scanning Microscopy
Flow Cytometry
Cells
Scanning electron microscopy
Antibodies
Fibers

Keywords

  • Confocal microscopy
  • Cytoskeleton
  • Microtubuli
  • Tetrahymena
  • Tubulin modification

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Effects of sodium fluoride (NaF) on the cilia and microtubular system of Tetrahymena. / Kovács, P.; Pállinger, E.; Csaba, G.

In: Cell Biochemistry and Function, Vol. 26, No. 5, 07.2008, p. 591-597.

Research output: Contribution to journalArticle

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