EINFLUSS VON SIN 1 AUF WECHSELWIRKUNGEN VON ENDOTHELZELLEN UND THROMBOZYTEN

Translated title of the contribution: Effects of SIN 1 on induced platelet aggregation in the presence of a human endothelial cell monolayer

C. M. Kirchmaier, I. Altorjay, O. Bellinger, H. K. Breddin

Research output: Contribution to journalArticle

Abstract

A new method has been developed to investigate the direct effect of endothelial cells on platelet aggregation in the presence of cultured confluent human EC monolayers. The insides of the disk shaped cuvettes are covered by human umbilical vein ECs. The cuvettes rotate in the light beam of a photometer. In these cuvettes the effect of different aggregation inducers was inhibited in a concentration-dependent manner, e.g. for collagen at 0.5 μg/ml, ADP at 5 x 10-7 M, epinephrine at 5 x 10-7 M and thrombin at 0.05 U/ml final concentration. (Platelet count 5 x 105/μl). Higher concentrations of the inducers led to irreversible platelet aggregation and were used for testing of antiplatelet drugs. In this system we detected a synergism between the antiaggregatory effect of the EC monolayer and that of SIN 1 (Cassella, Frankfurt/Main) the main metabolit of Molsidomine. 10 μg/ml PRP of SIN 1 alone partially inhibited platelet aggregation induced by 1 μg/ml collagen and 10-6 M ADP respectively in uncovered aggregation cuvettes. In the presence of an EC monolayer complete inhibition of platelet aggregation was obtained at a 5-fold final concentration of both inducers. After pretreatment of ECs with 1 mmol ASA over 30 min the antiaggregatory effect of SIN 1 decreased, but was more pronounced (50%) than in uncovered cuvettes (25%).

Original languageGerman
Pages (from-to)13-16
Number of pages4
JournalZeitschrift fur Kardiologie
Volume80
Issue numberSUPPL. 5
Publication statusPublished - 1991

Fingerprint

Platelet Aggregation
Endothelial Cells
Adenosine Diphosphate
Collagen
Molsidomine
Umbilical Veins
Platelet Aggregation Inhibitors
Platelet Count
Thrombin
Epinephrine
Light

Keywords

  • Endothelial cells
  • Molsidomine (SIN 1)
  • Platelet aggregation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Kirchmaier, C. M., Altorjay, I., Bellinger, O., & Breddin, H. K. (1991). EINFLUSS VON SIN 1 AUF WECHSELWIRKUNGEN VON ENDOTHELZELLEN UND THROMBOZYTEN. Zeitschrift fur Kardiologie, 80(SUPPL. 5), 13-16.

EINFLUSS VON SIN 1 AUF WECHSELWIRKUNGEN VON ENDOTHELZELLEN UND THROMBOZYTEN. / Kirchmaier, C. M.; Altorjay, I.; Bellinger, O.; Breddin, H. K.

In: Zeitschrift fur Kardiologie, Vol. 80, No. SUPPL. 5, 1991, p. 13-16.

Research output: Contribution to journalArticle

Kirchmaier, CM, Altorjay, I, Bellinger, O & Breddin, HK 1991, 'EINFLUSS VON SIN 1 AUF WECHSELWIRKUNGEN VON ENDOTHELZELLEN UND THROMBOZYTEN', Zeitschrift fur Kardiologie, vol. 80, no. SUPPL. 5, pp. 13-16.
Kirchmaier, C. M. ; Altorjay, I. ; Bellinger, O. ; Breddin, H. K. / EINFLUSS VON SIN 1 AUF WECHSELWIRKUNGEN VON ENDOTHELZELLEN UND THROMBOZYTEN. In: Zeitschrift fur Kardiologie. 1991 ; Vol. 80, No. SUPPL. 5. pp. 13-16.
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