Effects of regular exercise on neutrophil functions, oxidative stress parameters and antibody responses against 4-hydroxy-2-nonenal adducts in middle aged humans

Soichiro Sasaki, Takayuki Matsuura, Rei Takahashi, Takuya Iwasa, Hitoshi Watanabe, Kouji Shirai, Hideko Nakamoto, Sataro Goto, Shinya Akita, Yoshiro Kobayashi

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Abstract

Regular exercise has recognized health benefits, partly because it reportedly lowers the levels of the oxidation products of proteins and DNA at rest, in contrast with the effect of acute exercise. However, when we compared oxidative response markers in active middle-aged subjects with those in sedentary ones, the level of urinary 8-OHdG was higher in active subjects. Because neutrophils are the first line of defense against a variety of infectious diseases, we then compared the cell density, functions and apoptosis of neutrophils in active subjects with those in sedentary ones. The cell density of neutrophils and phagocytosis of opsonized zymosan by neutrophils were higher in active subjects, being similar with the reported effects of acute exercise. To determine any beneficial effects of oxidative stress in active subjects, we then compared the levels of antibodies against 4- hydroxy-2-nonenal adducts in active subjects with those in sedentary ones, because 4-hydroxy-2-nonenal is one of the most common bioactive aldehyde products of oxidative stress, and because the IgM class of antibodies against oxidized low-density lipoprotein is associated with atheroprotective properties. The level of the IgM but not the IgG class of antibodies against 4-hydroxy-2-nonenal adducts was higher in active subjects. Overall, this study revealed that our active middle-aged subjects showed both oxidative responses and a higher IgM response to reactive carbonyl derivatives, possibly providing a basis for a health benefit by exercise in our active subjects.

Original languageEnglish
Pages (from-to)60-71
Number of pages12
JournalExercise Immunology Review
Volume19
Publication statusPublished - Nov 25 2013

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Keywords

  • Anti-oxidative response
  • Antibodies against 4-hydroxy-2-nonenal adducts
  • Oxidative response
  • Regular exercise

ASJC Scopus subject areas

  • Immunology

Cite this

Sasaki, S., Matsuura, T., Takahashi, R., Iwasa, T., Watanabe, H., Shirai, K., Nakamoto, H., Goto, S., Akita, S., & Kobayashi, Y. (2013). Effects of regular exercise on neutrophil functions, oxidative stress parameters and antibody responses against 4-hydroxy-2-nonenal adducts in middle aged humans. Exercise Immunology Review, 19, 60-71.