Effects of polyethylene terephthalate on yeast alcohol dehydrogenase.

L. M. Simon, K. Heinrichova, I. Veszelka, B. Szajáni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Yeast alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.1) was adsorbed onto polyethylene terephthalate, a synthetic polymer. The effects of the polymer on the properties of the enzyme were studied. The specific activity of the bound enzyme on protein basis was only 1.2 per cent of the specific activity of the soluble enzyme. The optimum pH for the catalytic activity was strongly shifted toward acidic direction. The apparent temperature optimum of the bound enzyme was identical with that of the soluble form. The apparent Michaelis constants of the bound enzyme were higher for both ethanol and NAD+. The conformational stability of the enzyme against heat treatment and urea was decreased as a consequence of adsorption.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalActa Biochimica et Biophysica Hungarica
Volume25
Issue number1-2
Publication statusPublished - 1990

Fingerprint

Polyethylene Terephthalates
Alcohol Dehydrogenase
Enzymes
Polymers
Enzyme Stability
NAD
Adsorption
Urea
Ethanol
Hot Temperature
Catalyst activity
Heat treatment
Temperature
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Effects of polyethylene terephthalate on yeast alcohol dehydrogenase. / Simon, L. M.; Heinrichova, K.; Veszelka, I.; Szajáni, B.

In: Acta Biochimica et Biophysica Hungarica, Vol. 25, No. 1-2, 1990, p. 1-7.

Research output: Contribution to journalArticle

Simon, L. M. ; Heinrichova, K. ; Veszelka, I. ; Szajáni, B. / Effects of polyethylene terephthalate on yeast alcohol dehydrogenase. In: Acta Biochimica et Biophysica Hungarica. 1990 ; Vol. 25, No. 1-2. pp. 1-7.
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