Effects of pituitary beta-endorphin secretagogues on the concentration of beta-endorphin in rat cerebrospinal fluid

Evidence for a role of vasopressin in the regulation of brain beta-endorphin release

I. Barna, C. G J Fred Sweep, H. Dick Veldhuis, Victor M. Wiegant, David De Wied

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The concentration of P-endorphin-immunoreactivity (bE-IR) in cerebrospinal fluid (CSF) and plasma of rats was determined following intracerebroventricular (ICV) treatment of conscious animals with substances known to stimulate the release of PE and other pro-opiomelanocortin (POMC)-derived peptides at the level of the anteriorand intermediate lobes of the pituitary. The p-adrenoceptor agoinst isoproterenol (ISO) did not influence the concentration of PE-IR in CSF collected 5-60 min after ICV administration of doses ranging from 3 to 30,000 pg/rat. Plasma bE-IR levels, however, were significantly increased 20 min following ICV injection of 30,000 pg ISO. ICV treatment of animals with ovine corticotropin-releasing factor (CRF; 30-30,000 pg/rat) also did not affect CSF levels of bE-IR, whereas CRF in a dose of 30 pg significantly decreased, and in doses of 300-30,000 pg enhanced plasma bFE-IR concentrations as determined by 20 min following treatments. ICV injection of arginine8-vasopressin (AVP) in doses of 10-1,000 pg/rat dose-dependently elevated the bE-IR concentration in CSF without affecting plasma bE-IR levels. This AVP-induced increase in CSF PE-IR was maximal 20-35 min and bE-IR levels had returned to basal 60 min following treatment. The data indicate that AVP and not ISO and CRF is a stimulator of CSF levels of bE-IR. As bE-IR in CSF likely originates from brain POMC neurons, these results suggest the hot vasopressin may be a physiological regulator of brain POMC activity, and may act as a releasing factor for POMC-derived peptides in the brain.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalNeuroendocrinology
Volume51
Issue number1
DOIs
Publication statusPublished - 1990

Fingerprint

beta-Endorphin
Vasopressins
Cerebrospinal Fluid
Pro-Opiomelanocortin
Brain
Isoproterenol
Intermediate Pituitary Gland
Endorphins
Peptides
Injections
Corticotropin-Releasing Hormone
Therapeutics
Adrenergic Receptors
Sheep
Neurons

Keywords

  • B-Endorphin
  • Brain
  • Cerebrospinal fluid
  • Corticotropin-releasing factor
  • Isoproterenol
  • Pituitary
  • Plasma
  • Vasopressin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Effects of pituitary beta-endorphin secretagogues on the concentration of beta-endorphin in rat cerebrospinal fluid : Evidence for a role of vasopressin in the regulation of brain beta-endorphin release. / Barna, I.; Fred Sweep, C. G J; Dick Veldhuis, H.; Wiegant, Victor M.; Wied, David De.

In: Neuroendocrinology, Vol. 51, No. 1, 1990, p. 104-110.

Research output: Contribution to journalArticle

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