Effects of pituitary adenylate cyclase-activating polypeptide on the cyclooxygenase pathway of rat platelets and on platelet aggregation

Béla Kis, Zsófia Mezei, Géza Dancsó, János Pataricza, Árpád Gecse, Julius Gy Papp, Gyula Telegdy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Several data suggest that pituitary adenylate cyclase-activating polypeptide (PACAP) is involved in the regulation of local circulation. One possible role of PACAP in the regulation of circulation is that, it may modify the cyclooxygenase pathway of the arachidonate cascade in platelets. Our study was designed to study the effect of PACAP on the cyclooxygenase pathway of rat platelets and on platelet aggregation. PACAP (10-7 and 10- 6 M) significantly inhibited the cyclooxygenase pathway of platelets, mostly the thromboxane synthesis. Pretreatment with a PACAP receptor antagonist, PACAP(6-38), or with an inhibitor of protein kinase A, H-89, shows that the effects of PACAP on the cyclooxygenase pathway were diminished. In the aggregation studies, PACAP inhibited both the arachidonic acid-induced and the thrombin-induced platelet aggregation. It can be concluded that PACAP inhibits the cyclooxygenase pathway of rat platelets via a specific PACAP receptor-activated, cAMP-dependent pathway, and these effects of PACAP are involved in the inhibition of platelet aggregation.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalProstaglandins and Other Lipid Mediators
Volume58
Issue number2-4
DOIs
Publication statusPublished - Oct 1999

Keywords

  • Adenylate cyclase-activating polypeptide
  • Cyclooxygenase
  • Eicosanoids
  • PACAP
  • Pituitary
  • Platelet aggregation
  • Platelets
  • Rat platelet

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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