Effects of Pituitary Adenylate Cyclase Activating Polypeptide in Human Proximal Tubule Cells Against Gentamicin Toxicity

G. Horvath, D. Reglodi, P. Czetany, A. Illes, G. Reman, A. Fekete, G. Tóth, E. Laszlo, B. Opper

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nephrotoxicity by aminoglycoside antibiotics is a common cause of drug-induced nephropathy. Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide first isolated from hypothalamus, but later shown in widespread distribution. It exerts general cytoprotective effects in different cell types. Renoprotective effects of PACAP have been shown in various in vivo and in vitro experiments, but it is still not known whether it protects human proximal tubular cells against gentamicin toxicity. The aim of the present study was to investigate whether PACAP could influence the survival rate of human proximal tubular epithelial cells exposed to gentamicin treatment. For investigating its effect on cell survival after gentamicin treatment, cell viability was evaluated using MTT assay. Obtaining further insight into the background mechanism of gentamicin exposure and PACAP’s effect, expression of numerous kidney-related proteins was investigated using kidney biomarker array. Our results show that PACAP had protective effects against gentamicin-induced decreased cell viability, while it did not influence the proliferation activity of HK-2 cells. PACAP could counteract the expression-decreasing effect of gentamicin on dipeptidyl peptidase IV and vascular endothelial growth factor as assessed by kidney biomarker array. In summary, our present study could prove the protective effect of exogenous PACAP in gentamicin-induced nephrotoxicity.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalInternational Journal of Peptide Research and Therapeutics
DOIs
Publication statusAccepted/In press - Jan 4 2018

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Gentamicins
Toxicity
Cell Survival
Cells
Biomarkers
Kidney
Dipeptidyl Peptidase 4
Aminoglycosides
Antibiotics
Neuropeptides
Vascular Endothelial Growth Factor A
Hypothalamus
Assays
Epithelial Cells
Anti-Bacterial Agents
Proteins
Pharmaceutical Preparations

Keywords

  • Aminoglycoside
  • Cell survival
  • Cytoprotection
  • HK-2
  • Pituitary adenylate cyclase activating polypeptide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Bioengineering
  • Biochemistry
  • Molecular Medicine
  • Drug Discovery

Cite this

Effects of Pituitary Adenylate Cyclase Activating Polypeptide in Human Proximal Tubule Cells Against Gentamicin Toxicity. / Horvath, G.; Reglodi, D.; Czetany, P.; Illes, A.; Reman, G.; Fekete, A.; Tóth, G.; Laszlo, E.; Opper, B.

In: International Journal of Peptide Research and Therapeutics, 04.01.2018, p. 1-8.

Research output: Contribution to journalArticle

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