Effects of phospholipids on the binding of [3H]dihydroalprenolol to the β-adrenergic receptor of rabbit heart membranes

N. X. Thang, A. Borsodi, M. Wollemann

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Abstract

The specific binding of [3H]dihydroalprenolol (DHA) by rabbit heart membranes and by a solubilized Lubrol PX-free preparation amounted to 0.80 ± 0.06 pmoles/mg protein and 0.61 ± 0.07 pmoles/mg protein, respectively. The binding was stereospecific. Optimal solubilization was achieved with 1% Lubrol PX. The addition of total rabbit brain phospholipids significantly enhanced the specific [3H]DHA binding of the solubilized Lubrol PX-free receptor fraction. Although the number of binding sites (B(max)) increased in the presence of phospholipids, the dissociation and the K(d) constant of [3H]DHA in the solubilized Lubrol PX-free receptor fraction did not change.

Original languageEnglish
Pages (from-to)2791-2797
Number of pages7
JournalBiochemical Pharmacology
Volume29
Issue number20
DOIs
Publication statusPublished - 1980

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Dihydroalprenolol
Adrenergic Receptors
Phospholipids
Rabbits
Membranes
Brain
Proteins
Binding Sites
polidocanol

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of phospholipids on the binding of [3H]dihydroalprenolol to the β-adrenergic receptor of rabbit heart membranes. / Thang, N. X.; Borsodi, A.; Wollemann, M.

In: Biochemical Pharmacology, Vol. 29, No. 20, 1980, p. 2791-2797.

Research output: Contribution to journalArticle

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