Effects of orexin-monoaminergic interactions on oxytocin secretion in rat neurohypophyseal cell cultures

Tímea Ocskó, Márta Gálfi, Mariann Radács, Zsolt Molnár, Gyöngyi K. Kis, Kinga Rákosi, Andor H. Molnár, Ferenc László, Ferenc A. László, Csaba Varga

Research output: Contribution to journalArticle

5 Citations (Scopus)


The effects of orexin-monoaminergic compound interactions on oxytocin release were studied in 14-day rat neurohypophyseal cell cultures prepared by an enzymatic dissociation technique. The oxytocin contents of the supernatants were determined by radioimmunoassay. Following the administration of orexin-A or orexin-B in increasing doses, significant changes were not observed in the oxytocin content of the supernatant media. The oxytocin level increased substantially in response to adrenaline, noradrenaline, serotonin, histamine, dopamine or K + treatment. Preincubation with orexin-A or orexin-B reduced the adrenaline-, histamine- or serotonin-induced oxytocin level increases, but the oxytocin concentrations of the supernatant media remained above the control level. There was no significant difference in decreasing effect between orexin-A and orexin-B. Neither orexin-A nor orexin-B induced changes in oxytocin release following monoaminergic compound treatment. The results indicate that the changes in oxytocin secretion induced by the monoaminergic system can be directly influenced by the orexin system. The effects of orexin on oxytocin release can be antagonized by an orexin-1 receptor-specific antagonist. It may be presumed that the orexins can play a role in the pathogenetic process of metabolic diseases (e.g. obesity) by reducing the effects of increased oxytocin release caused by monoaminergic compounds. The interactions between the monoaminergic and orexin systems regarding oxytocin secretion occur at both the hypothalamic and the neurohypophyseal levels.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalRegulatory Peptides
Issue number1-3
Publication statusPublished - Apr 10 2012


  • Monoaminergic system
  • Neurohypophyseal cell culture
  • Orexin
  • Oxytocin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Effects of orexin-monoaminergic interactions on oxytocin secretion in rat neurohypophyseal cell cultures'. Together they form a unique fingerprint.

  • Cite this