Effects of nociceptin on the spread and seizure activity in the rat amygdala kindling model: Their correlations with 3H-leucyl-nociceptin binding

Liliana Carmona-Aparicio, Fernando Peña, A. Borsodi, Luisa Rocha

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effects with pretreatment with nociceptin (0.03-30 nmol, i.c.v.) were evaluated on the threshold for eliciting afterdischarge (ADT), generation and spread of seizure activity and postictal depression in rats with kindling stimulation. Nociceptin produced a decrease in ADT (32-45%) in rats with partial seizures (PS, stage II-III), and an increase (61-92%) in rats with generalized seizures (GS, kindled state). Nociceptin did not modify the behavioral changes, spike frequency and duration of afterdischarge elicited at ADT in both experimental groups. In rats with GS, nociceptin enhanced postictal depression (34-44%) evaluated with a recycling paradigm. Autoradiography experiments revealed enhanced nociceptin opioid receptor (NOP) binding in medial amygdala (22-26%), frontal (21-23%) and entorhinal (27-32%) cortices, and reduced binding in the substantia nigra pars compacta (28%) and medial central gray (29%) of rats with PS. The GS group displayed significant decreased NOP binding (40-70%) in most of the brain areas evaluated. These results suggest that nociceptin facilitates ictal activity in rats with PS, whereas in animals with GS, it induces inhibitory effects on ADT and enhances the postictal period. These effects correlate with significant changes in NOP binding.

Original languageEnglish
Pages (from-to)75-84
Number of pages10
JournalEpilepsy Research
Volume77
Issue number2-3
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Amygdala
Seizures
Opioid Receptors
Recycling
Autoradiography
nociceptin
Stroke
Brain
nociceptin receptor

Keywords

  • Afterdischarge threshold
  • Kindling
  • Nociceptin
  • Nociceptin receptor
  • Postictal depression

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Neurology

Cite this

Effects of nociceptin on the spread and seizure activity in the rat amygdala kindling model : Their correlations with 3H-leucyl-nociceptin binding. / Carmona-Aparicio, Liliana; Peña, Fernando; Borsodi, A.; Rocha, Luisa.

In: Epilepsy Research, Vol. 77, No. 2-3, 12.2007, p. 75-84.

Research output: Contribution to journalArticle

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