Effects of modulated microwave and X-ray irradiation on the activity and distribution of Ca2+-ATPase in small intestine epithelial cells

Z. Somosy, G. Thuroczy, G. J. Koteles, J. Kovács, W. C. De Bruijn, L. G. Friberg

Research output: Contribution to journalArticle

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Abstract

The distribution and activity of Ca2+-ATPase were investigated by histochemical methods in small intestine epithelial cells of mice following total body 2450 MHz low frequency (16 Hz) microwave and X-ray irradiation. In the control animals, enzyme activities were found in the brush border and on lateral membranes, including junctional areas of the cells. The enzyme activity of lateral membranes was inhibited by quercetin, a specific inhibitor of Ca2+-ATPase. Immediately after square modulated (16 Hz) 2450 MHz microwave irradiation at 1 mW/cm2 power densities, we observed a decreased activity of Ca2+-ATPase on the lateral membrane regions. The X- ray irradiation (1 Gy) induced a similar decrease of Ca2+ -ATPase activity which was reversible within 24 hours. '5 Gy' doses resulted in a decrease of enzyme activities on both apical and lateral membrane areas persisting up to 24 hours following irradiation.

Original languageEnglish
Pages (from-to)613-620
Number of pages8
JournalScanning Microscopy
Volume8
Issue number3
Publication statusPublished - 1994

Fingerprint

intestines
enzyme activity
Enzyme activity
Microwaves
Irradiation
membranes
Membranes
microwaves
X rays
irradiation
x rays
Microwave irradiation
brushes
Brushes
borders
inhibitors
mice
animals
radiant flux density
Animals

Keywords

  • Ca-ATPase activity
  • modulated microwave irradiation
  • transmission electron microscopy
  • X-radiation

ASJC Scopus subject areas

  • Instrumentation

Cite this

Effects of modulated microwave and X-ray irradiation on the activity and distribution of Ca2+-ATPase in small intestine epithelial cells. / Somosy, Z.; Thuroczy, G.; Koteles, G. J.; Kovács, J.; De Bruijn, W. C.; Friberg, L. G.

In: Scanning Microscopy, Vol. 8, No. 3, 1994, p. 613-620.

Research output: Contribution to journalArticle

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