Effects of mistletoe extract on murine thymocytes in vivo and on glucocorticoid-induced cell count reduction

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Abstract

Background: Mistletoe extracts are widely used in cancer patients due to their cytostatic and immunomodulatory effects. Essential components include mistletoe lectins which act as biomodulators with proinflammatory and apoptosis-inducing effects. This study investigates the acute and long-term effects of standardized mistletoe extract (Iscador® M spec 5 mg) on thymocyte subpopulations and peripheral T-cells using a murine (Balb/c) model. Materials and Methods: Using cell surface CD4/CD8 staining and flow cytometry, we followed the changes in CD4-CD8- double-negative (DN), CD4+CD8+ double-positive (DP) and CD4+ or CD8+ single-positive (SP) T-cells 24 h after single or repeated injections of 3 different dilutions (1:12, 1:60, 1:300) corresponding to 2.1, 0.42 and 0.08 mg/kg of Iscador. Thymocyte apoptosis was detected by flow cytometry using Annexin V and propidium iodide. Results: 24 h after a single injection of the 2 lower doses, the number of DN thymocytes increased significantly with an enhanced ratio of apoptotic cells. Following administration of the lowest dose, in peripheral blood the CD4+/CD8+ ratio was elevated. In the long-term trial, Balb/c mice were treated twice a week with 3 different doses of Iscador +/- 20 mg/kg of dexamethasone (DX), resulting in significantly enhanced DN thymocytes and elevated levels of apoptotic cells after treatment with the 2 lower doses. Iscador also inhibited the DX-induced reduction in the thymic DN cell count, as well as the DX-induced decrease in the CD4+/CD8+ ratio and CD4+ in the peripheral blood. Conclusion: Our results suggest that standardized mistletoe extract modulates proliferation and apoptosis of thymocytes in a dose-dependent manner and may act lymphoprotective during DX treatment.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalForschende Komplementarmedizin
Volume13
Issue number1
DOIs
Publication statusPublished - Mar 2006

Fingerprint

viscum album peptide
Mistletoe
Thymocytes
Glucocorticoids
Cell Count
Dexamethasone
CD4-CD8 Ratio
Apoptosis
Flow Cytometry
T-Lymphocytes
Injections
Propidium
Annexin A5
Immunologic Factors
Cytostatic Agents
Lectins
Staining and Labeling
Therapeutics

Keywords

  • Apoptosis
  • Glucocorticoids
  • Mistletoe extract
  • Thymus

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

@article{d3fb1942e3ed4cd2bdffc0ee33dcf0b5,
title = "Effects of mistletoe extract on murine thymocytes in vivo and on glucocorticoid-induced cell count reduction",
abstract = "Background: Mistletoe extracts are widely used in cancer patients due to their cytostatic and immunomodulatory effects. Essential components include mistletoe lectins which act as biomodulators with proinflammatory and apoptosis-inducing effects. This study investigates the acute and long-term effects of standardized mistletoe extract (Iscador{\circledR} M spec 5 mg) on thymocyte subpopulations and peripheral T-cells using a murine (Balb/c) model. Materials and Methods: Using cell surface CD4/CD8 staining and flow cytometry, we followed the changes in CD4-CD8- double-negative (DN), CD4+CD8+ double-positive (DP) and CD4+ or CD8+ single-positive (SP) T-cells 24 h after single or repeated injections of 3 different dilutions (1:12, 1:60, 1:300) corresponding to 2.1, 0.42 and 0.08 mg/kg of Iscador. Thymocyte apoptosis was detected by flow cytometry using Annexin V and propidium iodide. Results: 24 h after a single injection of the 2 lower doses, the number of DN thymocytes increased significantly with an enhanced ratio of apoptotic cells. Following administration of the lowest dose, in peripheral blood the CD4+/CD8+ ratio was elevated. In the long-term trial, Balb/c mice were treated twice a week with 3 different doses of Iscador +/- 20 mg/kg of dexamethasone (DX), resulting in significantly enhanced DN thymocytes and elevated levels of apoptotic cells after treatment with the 2 lower doses. Iscador also inhibited the DX-induced reduction in the thymic DN cell count, as well as the DX-induced decrease in the CD4+/CD8+ ratio and CD4+ in the peripheral blood. Conclusion: Our results suggest that standardized mistletoe extract modulates proliferation and apoptosis of thymocytes in a dose-dependent manner and may act lymphoprotective during DX treatment.",
keywords = "Apoptosis, Glucocorticoids, Mistletoe extract, Thymus",
author = "T. Hajt{\'o} and T. Berki and L. P{\'a}link{\'a}s and F. Boldizs{\'a}r and P. N{\'e}meth",
year = "2006",
month = "3",
doi = "10.1159/000090265",
language = "English",
volume = "13",
pages = "22--27",
journal = "Forschende Komplementarmedizin",
issn = "1661-4119",
publisher = "S. Karger AG",
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TY - JOUR

T1 - Effects of mistletoe extract on murine thymocytes in vivo and on glucocorticoid-induced cell count reduction

AU - Hajtó, T.

AU - Berki, T.

AU - Pálinkás, L.

AU - Boldizsár, F.

AU - Németh, P.

PY - 2006/3

Y1 - 2006/3

N2 - Background: Mistletoe extracts are widely used in cancer patients due to their cytostatic and immunomodulatory effects. Essential components include mistletoe lectins which act as biomodulators with proinflammatory and apoptosis-inducing effects. This study investigates the acute and long-term effects of standardized mistletoe extract (Iscador® M spec 5 mg) on thymocyte subpopulations and peripheral T-cells using a murine (Balb/c) model. Materials and Methods: Using cell surface CD4/CD8 staining and flow cytometry, we followed the changes in CD4-CD8- double-negative (DN), CD4+CD8+ double-positive (DP) and CD4+ or CD8+ single-positive (SP) T-cells 24 h after single or repeated injections of 3 different dilutions (1:12, 1:60, 1:300) corresponding to 2.1, 0.42 and 0.08 mg/kg of Iscador. Thymocyte apoptosis was detected by flow cytometry using Annexin V and propidium iodide. Results: 24 h after a single injection of the 2 lower doses, the number of DN thymocytes increased significantly with an enhanced ratio of apoptotic cells. Following administration of the lowest dose, in peripheral blood the CD4+/CD8+ ratio was elevated. In the long-term trial, Balb/c mice were treated twice a week with 3 different doses of Iscador +/- 20 mg/kg of dexamethasone (DX), resulting in significantly enhanced DN thymocytes and elevated levels of apoptotic cells after treatment with the 2 lower doses. Iscador also inhibited the DX-induced reduction in the thymic DN cell count, as well as the DX-induced decrease in the CD4+/CD8+ ratio and CD4+ in the peripheral blood. Conclusion: Our results suggest that standardized mistletoe extract modulates proliferation and apoptosis of thymocytes in a dose-dependent manner and may act lymphoprotective during DX treatment.

AB - Background: Mistletoe extracts are widely used in cancer patients due to their cytostatic and immunomodulatory effects. Essential components include mistletoe lectins which act as biomodulators with proinflammatory and apoptosis-inducing effects. This study investigates the acute and long-term effects of standardized mistletoe extract (Iscador® M spec 5 mg) on thymocyte subpopulations and peripheral T-cells using a murine (Balb/c) model. Materials and Methods: Using cell surface CD4/CD8 staining and flow cytometry, we followed the changes in CD4-CD8- double-negative (DN), CD4+CD8+ double-positive (DP) and CD4+ or CD8+ single-positive (SP) T-cells 24 h after single or repeated injections of 3 different dilutions (1:12, 1:60, 1:300) corresponding to 2.1, 0.42 and 0.08 mg/kg of Iscador. Thymocyte apoptosis was detected by flow cytometry using Annexin V and propidium iodide. Results: 24 h after a single injection of the 2 lower doses, the number of DN thymocytes increased significantly with an enhanced ratio of apoptotic cells. Following administration of the lowest dose, in peripheral blood the CD4+/CD8+ ratio was elevated. In the long-term trial, Balb/c mice were treated twice a week with 3 different doses of Iscador +/- 20 mg/kg of dexamethasone (DX), resulting in significantly enhanced DN thymocytes and elevated levels of apoptotic cells after treatment with the 2 lower doses. Iscador also inhibited the DX-induced reduction in the thymic DN cell count, as well as the DX-induced decrease in the CD4+/CD8+ ratio and CD4+ in the peripheral blood. Conclusion: Our results suggest that standardized mistletoe extract modulates proliferation and apoptosis of thymocytes in a dose-dependent manner and may act lymphoprotective during DX treatment.

KW - Apoptosis

KW - Glucocorticoids

KW - Mistletoe extract

KW - Thymus

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JO - Forschende Komplementarmedizin

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SN - 1661-4119

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