Effects of low-dose radiation on the immune system of mice after total-body irradiation

Enikö Noémi Bogdándi, Andrea Balogh, Nikolett Felgyinszki, Tünde Szatmári, Eszter Persa, Guido Hildebrandt, Géza Sáfrány, Katalin Lumniczky

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Abstract

The effects of acute exposure to low- and high-dose radiation on the quantitative and functional parameters of the immune system were analyzed. C57BL/6 mice were irradiated with different doses of γ radiation (0.01, 0.05, 0.1, 0.5 and 2 Gy) and splenocytes were isolated at various times. Alterations in the distribution and surviving fraction of splenocyte subsets such as CD4 and CD8 T lymphocytes, regulatory T cells (Treg), natural killer (NK) cells, dendritic cells (DCs) and B lymphocytes were analyzed by flow cytometry. Apoptosis frequency was quantified by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method 4 h after irradiation. Cytokine expression was investigated by real-time reverse transcription-polymerase chain reaction (RT-PCR). Low doses decreased apoptosis in the splenocyte subpopulations studied most prominently in NK cells and DCs. Exposure to 2 Gy increased apoptosis in all splenocyte subpopulations; B cells were the most sensitive and NK cells and DCs the least sensitive. The lowest cell numbers were measured 3 days after irradiation, with minor changes by day 7. CD8 and B cells were rather resistant to low doses but were very sensitive to 2 Gy, while NK cells, DCs and Treg cells were much more resistant to high doses. Expression of the T-helper 1 (Th1)- and helper 2 (Th2)-type cytokines decreased after low doses and increased after high doses. Interleukin 6 (IL-6) reacted at early times and IL-10 at later times. IL-5 levels were consistently elevated. These data highlight the differences in the responses of different splenocyte subpopulations to low- and high-dose radiation.

Original languageEnglish
Pages (from-to)480-489
Number of pages10
JournalRadiation Research
Volume174
Issue number4
DOIs
Publication statusPublished - Oct 2010

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ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Bogdándi, E. N., Balogh, A., Felgyinszki, N., Szatmári, T., Persa, E., Hildebrandt, G., Sáfrány, G., & Lumniczky, K. (2010). Effects of low-dose radiation on the immune system of mice after total-body irradiation. Radiation Research, 174(4), 480-489. https://doi.org/10.1667/RR2160.1