Effects of laser treatment on the expression of cytosolic proteins in the synovium of patients with osteoarthritis

Klára Barabás, József Bakos, Zsuzsanna Zeitler, G. Bálint, Erzsébet Nagy, Tamás Lakatos, K. Kékesi, Lajos Gáspár, Z. Szekanecz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Results: Eleven proteins showing altered expression due to laser irradiation were identified. There were three patients whose tissue samples demonstrated a significant increase (P

Study Design/Materials and methods: The synovial membrane samples removed from the knees of 6 OA patients were irradiated ex vivo using near infrared diode laser (807-811 nm; 25 J/cm2). An untreated sample taken from the same patient served as control. Synovial protein separation and identification were performed by two dimensional differential gel electrophoresis and mass spectrometry, respectively.

Conclusions: A single diode laser irradiation of the synovial samples of patients with osteoarthritis can statistically significantly alter the expression of some proteins in vitro. These findings provide some more evidence for biological efficacy of LLLT treatment, used for osteoarthritis. Lasers Surg. Med. 46:644-649, 2014.

Background and Objective: Low level laser therapy (LLLT) has been developed for non-invasive treatment of joint diseases. We have previously shown that LLLT influenced synovial protein expression in rheumatoid arthritis (RA). The aim of this study was to assess the effects of laser irradiation on osteoarthritic (OA) synovial protein expression.

Original languageEnglish
Pages (from-to)644-649
Number of pages6
JournalLasers in Surgery and Medicine
Volume46
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Synovial Membrane
Osteoarthritis
Lasers
Semiconductor Lasers
Proteins
Therapeutics
Joint Diseases
Electrophoresis, Gel, Two-Dimensional
Mass Spectrometry
Rheumatoid Arthritis
Knee
Low-Level Light Therapy

Keywords

  • Ex vivo
  • Laser therapy
  • Proteomics
  • Synovial membrane

ASJC Scopus subject areas

  • Surgery
  • Dermatology
  • Medicine(all)

Cite this

Effects of laser treatment on the expression of cytosolic proteins in the synovium of patients with osteoarthritis. / Barabás, Klára; Bakos, József; Zeitler, Zsuzsanna; Bálint, G.; Nagy, Erzsébet; Lakatos, Tamás; Kékesi, K.; Gáspár, Lajos; Szekanecz, Z.

In: Lasers in Surgery and Medicine, Vol. 46, No. 8, 2014, p. 644-649.

Research output: Contribution to journalArticle

Barabás, Klára ; Bakos, József ; Zeitler, Zsuzsanna ; Bálint, G. ; Nagy, Erzsébet ; Lakatos, Tamás ; Kékesi, K. ; Gáspár, Lajos ; Szekanecz, Z. / Effects of laser treatment on the expression of cytosolic proteins in the synovium of patients with osteoarthritis. In: Lasers in Surgery and Medicine. 2014 ; Vol. 46, No. 8. pp. 644-649.
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