Effects of kisspeptin on diabetic rat platelets

Zsófia Mezei, Sándor Váczi, Viktória Török, Csaba Stumpf, Rita Ónody, Imre Földesi, Gyula Szabó

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1 Citation (Scopus)

Abstract

Hyperglycemia, hyperlipidemia, and free radicals result in platelet activation and atherogenesis. Kisspeptin (KP) is able to regulate metabolism, hemostasis, and the development of atherosclerosis. We examined whether platelet aggregation of streptozotocin-induced diabetic rats depends on the inducer type and if KP-13 and RF-9 (a kisspeptin receptor modifier) can influence platelet function. We measured the speed and the maximum of aggregation, along with the area under the curve. Serum glucose and calcium levels and urine formation of diabetic animals increased, while the body mass and platelet count decreased. Collagen was the most effective inducer of platelet aggregation. The aggregability of nondiabetic platelets was elevated in the presence of 5 × 10−8 mol/L KP-13. This effect was less expressed in diabetic animals. The effectivity of RF-9 was stronger than that of KP-13 in nondiabetic platelets, however it was ineffective in diabetic animals. RF-9 pre-treatment did not change the effects of 5 × 10−8 mol/L KP-13 in either animal group. The in vivo activation of diabetic platelets, which may be due to elevated serum calcium, induces thrombocytopenia and may lead to reduced in vitro aggregability. We could not demonstrate the antagonistic effect of RF-9 against KP-13 in isolated platelets.

Original languageEnglish
Pages (from-to)1319-1326
Number of pages8
JournalCanadian journal of physiology and pharmacology
Volume95
Issue number11
DOIs
Publication statusPublished - Jan 1 2017

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Keywords

  • Aggregation
  • Aggregometry
  • Diabetes
  • Kisspeptin
  • Platelets
  • RF-9
  • Streptozotocin

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Mezei, Z., Váczi, S., Török, V., Stumpf, C., Ónody, R., Földesi, I., & Szabó, G. (2017). Effects of kisspeptin on diabetic rat platelets. Canadian journal of physiology and pharmacology, 95(11), 1319-1326. https://doi.org/10.1139/cjpp-2017-0036