Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats

Krisztina Csabafi, M. Jászberényi, Zsolt Bagosi, Nándor Lipták, G. Telegdy

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Kisspeptin is a mammalian amidated neurohormone, which belongs to the RF-amide peptide family and is known for its key role in reproduction. However, in contrast with the related members of the RF-amide family, little information is available regarding its role in the stress-response. With regard to the recent data suggesting kisspeptin neuronal projections to the paraventricular nucleus, in the present experiments we investigated the effect of kisspeptin-13 (KP-13), an endogenous derivative of kisspeptin, on the hypothalamus-pituitary-adrenal (HPA) axis, motor behavior and thermoregulatory function. The peptide was administered intracerebroventricularly (icv.) in different doses (0.5-2 μg) to adult male Sprague-Dawley rats, the behavior of which was then observed by means of telemetry, open field and elevated plus maze tests. Additionally, plasma concentrations of corticosterone were measured in order to assess the influence of KP-13 on the HPA system. The effects on core temperature were monitored continuously via telemetry. The results demonstrated that KP-13 stimulated the horizontal locomotion (square crossing) in the open field test and decreased the number of entries into and the time spent in the open arms during the elevated plus maze tests. The peptide also caused marked elevations in the spontaneous locomotor activity and the core temperature recorded by the telemetric system, and significantly increased the basal corticosterone level. In conclusion, our data indicate that icv. administered KP-13 stimulates the HPA axis, induces hyperthermia, activates motor behavior and causes anxiety in rats.

Original languageEnglish
Pages (from-to)56-61
Number of pages6
JournalBehavioural Brain Research
Volume241
Issue number1
DOIs
Publication statusPublished - Mar 5 2013

Fingerprint

Kisspeptins
Body Temperature Regulation
Locomotion
Anxiety
Hypothalamus
Telemetry
Corticosterone
Amides
Peptides
Pituitary-Adrenal System
Temperature
Paraventricular Hypothalamic Nucleus
Reproduction
Neurotransmitter Agents
Sprague Dawley Rats
Fever

Keywords

  • Hyperthermia
  • Hypothalamic-pituitary-adrenal axis
  • Kisspeptin
  • Locomotor activity

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats. / Csabafi, Krisztina; Jászberényi, M.; Bagosi, Zsolt; Lipták, Nándor; Telegdy, G.

In: Behavioural Brain Research, Vol. 241, No. 1, 05.03.2013, p. 56-61.

Research output: Contribution to journalArticle

@article{5495fd9c1b984092a7ce8db44ec081c1,
title = "Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats",
abstract = "Kisspeptin is a mammalian amidated neurohormone, which belongs to the RF-amide peptide family and is known for its key role in reproduction. However, in contrast with the related members of the RF-amide family, little information is available regarding its role in the stress-response. With regard to the recent data suggesting kisspeptin neuronal projections to the paraventricular nucleus, in the present experiments we investigated the effect of kisspeptin-13 (KP-13), an endogenous derivative of kisspeptin, on the hypothalamus-pituitary-adrenal (HPA) axis, motor behavior and thermoregulatory function. The peptide was administered intracerebroventricularly (icv.) in different doses (0.5-2 μg) to adult male Sprague-Dawley rats, the behavior of which was then observed by means of telemetry, open field and elevated plus maze tests. Additionally, plasma concentrations of corticosterone were measured in order to assess the influence of KP-13 on the HPA system. The effects on core temperature were monitored continuously via telemetry. The results demonstrated that KP-13 stimulated the horizontal locomotion (square crossing) in the open field test and decreased the number of entries into and the time spent in the open arms during the elevated plus maze tests. The peptide also caused marked elevations in the spontaneous locomotor activity and the core temperature recorded by the telemetric system, and significantly increased the basal corticosterone level. In conclusion, our data indicate that icv. administered KP-13 stimulates the HPA axis, induces hyperthermia, activates motor behavior and causes anxiety in rats.",
keywords = "Hyperthermia, Hypothalamic-pituitary-adrenal axis, Kisspeptin, Locomotor activity",
author = "Krisztina Csabafi and M. J{\'a}szber{\'e}nyi and Zsolt Bagosi and N{\'a}ndor Lipt{\'a}k and G. Telegdy",
year = "2013",
month = "3",
day = "5",
doi = "10.1016/j.bbr.2012.11.039",
language = "English",
volume = "241",
pages = "56--61",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats

AU - Csabafi, Krisztina

AU - Jászberényi, M.

AU - Bagosi, Zsolt

AU - Lipták, Nándor

AU - Telegdy, G.

PY - 2013/3/5

Y1 - 2013/3/5

N2 - Kisspeptin is a mammalian amidated neurohormone, which belongs to the RF-amide peptide family and is known for its key role in reproduction. However, in contrast with the related members of the RF-amide family, little information is available regarding its role in the stress-response. With regard to the recent data suggesting kisspeptin neuronal projections to the paraventricular nucleus, in the present experiments we investigated the effect of kisspeptin-13 (KP-13), an endogenous derivative of kisspeptin, on the hypothalamus-pituitary-adrenal (HPA) axis, motor behavior and thermoregulatory function. The peptide was administered intracerebroventricularly (icv.) in different doses (0.5-2 μg) to adult male Sprague-Dawley rats, the behavior of which was then observed by means of telemetry, open field and elevated plus maze tests. Additionally, plasma concentrations of corticosterone were measured in order to assess the influence of KP-13 on the HPA system. The effects on core temperature were monitored continuously via telemetry. The results demonstrated that KP-13 stimulated the horizontal locomotion (square crossing) in the open field test and decreased the number of entries into and the time spent in the open arms during the elevated plus maze tests. The peptide also caused marked elevations in the spontaneous locomotor activity and the core temperature recorded by the telemetric system, and significantly increased the basal corticosterone level. In conclusion, our data indicate that icv. administered KP-13 stimulates the HPA axis, induces hyperthermia, activates motor behavior and causes anxiety in rats.

AB - Kisspeptin is a mammalian amidated neurohormone, which belongs to the RF-amide peptide family and is known for its key role in reproduction. However, in contrast with the related members of the RF-amide family, little information is available regarding its role in the stress-response. With regard to the recent data suggesting kisspeptin neuronal projections to the paraventricular nucleus, in the present experiments we investigated the effect of kisspeptin-13 (KP-13), an endogenous derivative of kisspeptin, on the hypothalamus-pituitary-adrenal (HPA) axis, motor behavior and thermoregulatory function. The peptide was administered intracerebroventricularly (icv.) in different doses (0.5-2 μg) to adult male Sprague-Dawley rats, the behavior of which was then observed by means of telemetry, open field and elevated plus maze tests. Additionally, plasma concentrations of corticosterone were measured in order to assess the influence of KP-13 on the HPA system. The effects on core temperature were monitored continuously via telemetry. The results demonstrated that KP-13 stimulated the horizontal locomotion (square crossing) in the open field test and decreased the number of entries into and the time spent in the open arms during the elevated plus maze tests. The peptide also caused marked elevations in the spontaneous locomotor activity and the core temperature recorded by the telemetric system, and significantly increased the basal corticosterone level. In conclusion, our data indicate that icv. administered KP-13 stimulates the HPA axis, induces hyperthermia, activates motor behavior and causes anxiety in rats.

KW - Hyperthermia

KW - Hypothalamic-pituitary-adrenal axis

KW - Kisspeptin

KW - Locomotor activity

UR - http://www.scopus.com/inward/record.url?scp=84871649418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871649418&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2012.11.039

DO - 10.1016/j.bbr.2012.11.039

M3 - Article

C2 - 23219969

AN - SCOPUS:84871649418

VL - 241

SP - 56

EP - 61

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1

ER -