Effects of intranasal phototherapy on nasal mucosa in patients with allergic rhinitis

A. Koreck, A. Szechenyi, M. Morocz, A. Cimpean, Zs Bella, E. Garaczi, M. Raica, T. R. Olariu, I. Raskó, L. Kemény

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Rationale: Rhinophototherapy has been shown to be effective in the treatment of allergic rhinitis. Considering that phototherapy with ultraviolet light (UV) induces DNA damage, it is of outstanding importance to evaluate the damage and repair process in human nasal mucosa. Methods: We have investigated eight patients undergoing intranasal phototherapy using a modified Comet assay technique and by staining nasal cytology samples for cyclobutane pyrimidine dimers (CPDs), which are UV specific photoproducts. Results: Immediately after last treatment Comet assay of nasal cytology samples showed a significant increase in DNA damage compared to baseline. Ten days after the last irradiation a significant decrease in DNA damage was observed compared to data obtained immediately after finishing the treatment protocol. Difference between baseline and 10 days after last treatment was not statistically significant. Two months after ending therapy, DNA damage detected by Comet assay in patients treated with intranasal phototherapy was similar with that of healthy individuals. None of the samples collected before starting intranasal phototherapy stained positive for CPDs. In all samples collected immediately after last treatment strong positive staining for CPDs was detected. The number of positive cells significantly decreased 10 days after last treatment, but residual positive staining was present in all the examined samples. This finding is consistent with data reported in skin samples after UV irradiation. Cytology samples examined two months after ending therapy contained no CPD positive cells. Conclusion: Our results suggest that UV damage induced by intranasal phototherapy is efficiently repaired in nasal mucosa.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume89
Issue number2-3
DOIs
Publication statusPublished - Dec 14 2007

Fingerprint

Pyrimidine Dimers
nasal mucosa
Phototherapy
rhinitis
Nasal Mucosa
Cytology
Dimers
DNA
cyclobutane
pyrimidines
Assays
cytology
Ultraviolet Rays
damage
ultraviolet radiation
DNA damage
DNA Damage
Comet Assay
staining
deoxyribonucleic acid

Keywords

  • DNA damage
  • Nasal mucosa
  • Phototherapy

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Effects of intranasal phototherapy on nasal mucosa in patients with allergic rhinitis. / Koreck, A.; Szechenyi, A.; Morocz, M.; Cimpean, A.; Bella, Zs; Garaczi, E.; Raica, M.; Olariu, T. R.; Raskó, I.; Kemény, L.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 89, No. 2-3, 14.12.2007, p. 163-169.

Research output: Contribution to journalArticle

Koreck, A. ; Szechenyi, A. ; Morocz, M. ; Cimpean, A. ; Bella, Zs ; Garaczi, E. ; Raica, M. ; Olariu, T. R. ; Raskó, I. ; Kemény, L. / Effects of intranasal phototherapy on nasal mucosa in patients with allergic rhinitis. In: Journal of Photochemistry and Photobiology, B: Biology. 2007 ; Vol. 89, No. 2-3. pp. 163-169.
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