Effects of human trophoblast-induced interferons on the expression of proto-oncogenes c-fms/CSF-1R, EGF-R and c-erbB2 in invasive and non-invasive trophoblast

G. Aboagye-Mathiesen, M. Zdravkovic, F. Tóth, P. Ebbesen

Research output: Contribution to journalArticle

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Abstract

The human cytotrophoblast is the first fetal cell type to arise during embryogenesis and differentiate along two pathways to the invasive (extravillous) and non-invasive (villous) populations. The non-invasive villous trophoblast differentiate morphologically acid biochemically to form terminally differentiated multinucleated syncytial trophoblast. First trimester invasive and non-invasive trophoblast were isolated from human placentae (5-12 weeks) and were cultured in vitro. The villous trophoblast cells differentiated in vitro to form aggregated syncytial cells which was associated with increased expression of epidermal growth factor receptor (EGF-R). The invasive trophoblast cells expressed colony-stimulating factor receptor (c-fms/CSF-1R) and c-erbB2 proteins but low levels of EGF-R. We studied the effects of human trophoblast-induced interferon (IFN)-α/β on the expression of c-fms/CSF-1R, EGF-R and c-erbB2 whose ligands are reported to be involved in the regulation of growth and differentiation of normal invasive and non-invasive trophoblast cells. Human trophoblast-induced IFN-α/β (100 IU/ml) reduced the expression of EGF-R in both invasive and non-invasive trophoblast cells as determined by quantitative enzyme-linked immunosorbant assay ('ELISA) and western immunoblot methods. The same amount of IFN activity reduced the expression of c-fms/CSF-1R and c-erbB2 proto-oncogene products in invasive trophoblast cells. These results may suggest a possible role of trophoblast-induced IFNs in the regulation of normal trophoblast growth, differentiation and function.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalPlacenta
Volume18
Issue number2-3
DOIs
Publication statusPublished - 1997

Fingerprint

fms Genes
Trophoblasts
Epidermal Growth Factor Receptor
Interferons
Colony-Stimulating Factor Receptors
Proto-Oncogenes
Oncogene Proteins

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Effects of human trophoblast-induced interferons on the expression of proto-oncogenes c-fms/CSF-1R, EGF-R and c-erbB2 in invasive and non-invasive trophoblast. / Aboagye-Mathiesen, G.; Zdravkovic, M.; Tóth, F.; Ebbesen, P.

In: Placenta, Vol. 18, No. 2-3, 1997, p. 155-161.

Research output: Contribution to journalArticle

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