Effects of glucocorticoid agonist and antagonist on the pathogenesis of L-arginine-induced acute pancreatitis in rat

Attila Paszt, Katalin Éder, Annamária Szabolcs, László Tiszlavicz, György Lázár, Ernö Duda, Tamás Takács, György Lázár

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

OBJECTIVES: To investigate the consequences of treatment with an exogenous glucocorticoid agonist (methylprednisolone) and antagonist (RU-38486) on the local and systemic responses in l-arginine-induced acute pancreatitis in rats. METHODS: The methylprednisolone and RU-38486 were administered just before pancreatitis induction. Plasma amylase activity, interleukin 6 activity, pancreatic weight/body weight ratio, plasma macrophage migration inhibitory factor (MIF) concentration, and pancreatic nuclear transcription factor (NF) κB activity were determined. The extents of pancreas, liver, and lung injuries were assessed by histology. RESULTS: Acute pancreatitis resulted in NF-κB activation and proinflammatory cytokine release in rats. In the glucocorticoid agonist group, plasma amylase and interleukin 6 levels were significantly decreased as compared with those of RU-38486 and nontreated groups. Antagonist treatment led to significantly higher MIF production at 8 and 12 hours after l-arginine injection as compared with the agonist-treated and nontreated groups. Glucocorticoid agonist treatment significantly decreased the level of NF-κB 24 hours after pancreatitis induction. Histological investigations showed protective effect of agonist treatment on acute pancreatitis-induced tissue damage in the pancreas and lung. CONCLUSIONS: These results corroborated the importance of MIF in acute pancreatitis. The glucocorticoid-dependent mechanisms seem to play a crucial role in the control of the inflammatory response and tissue damage in l-arginine-induced experimental acute pancreatitis.

Original languageEnglish
Pages (from-to)369-376
Number of pages8
JournalPancreas
Volume36
Issue number4
DOIs
Publication statusPublished - May 1 2008

Keywords

  • Experimental pancreatitis
  • Glucocorticoid hormones
  • MIF
  • NF-κB
  • Organ failure

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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