Effects of galactomannan as carbon source on production of α-galactosidase by Thermomyces lanuginosus: Fermentation, purification and partial characterisation

Lilla Svastits-Dücso, Quang D. Nguyen, Dominika Dóra Lefler, Judit M. Rezessy-Szabó

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Thermophilic fungus Thermomyces lanuginosus CBS 395.62/b strain is able to grow and synthesise extracellular α-galactosidase in media containing galactomannan such as locust bean gum (LBG) or guar gum (GG). Production of extracellular α-galactosidase was enhanced from 1.2 U/mL to 4-6 U/mL meaning about 3-5 times increase by optimisation of medium composition. This enzyme was purified to homogeneity by partial precipitation with 2-propanol and different liquid chromatographical steps. The developed purification protocol yielded 22% of enzyme activity with 900 purified fold. Molecular mass of the purified α-galactosidase enzyme was estimated to be 53 kDa. Maximal catalytic activity of the enzyme was obtained in the acidic pH range between pH 4.6 and 4.8 and in the temperature range 60-66 °C. More than 95% of enzyme activity was remaining after 1-day incubation at 70 °C and on pH in the range from 4.0 to 7.0. The enzyme activity was significantly stimulated by Mg2+, Mn2+ and K+ ions, while considerably inhibited by the presence of Ca2+, Ag+ and Hg2+.

Original languageEnglish
Pages (from-to)367-371
Number of pages5
JournalEnzyme and Microbial Technology
Volume45
Issue number5
DOIs
Publication statusPublished - Nov 5 2009

Fingerprint

Galactosidases
Enzyme activity
Fermentation
Purification
guar gum
Carbon
Enzymes
2-Propanol
Molecular mass
Propanol
Fungi
Catalyst activity
Ions
Liquids
Chemical analysis
galactomannan
Temperature

Keywords

  • α-Galactosidase
  • Galactomannan
  • Thermomyces lanuginosus
  • Thermophilic fungus

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Effects of galactomannan as carbon source on production of α-galactosidase by Thermomyces lanuginosus : Fermentation, purification and partial characterisation. / Svastits-Dücso, Lilla; Nguyen, Quang D.; Lefler, Dominika Dóra; Rezessy-Szabó, Judit M.

In: Enzyme and Microbial Technology, Vol. 45, No. 5, 05.11.2009, p. 367-371.

Research output: Contribution to journalArticle

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