Effects of drought on water content and photosynthetic parameters in potato plants expressing the trehalose-6-phosphate synthase gene of Saccharomyces cerevisiae

Ibolya Stiller, Sándor Dulai, Mihály Kondrák, Réka Tarnai, László Szabó, O. Toldi, Z. Bánfalvi

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Two transgenic potato lines, T1 and T2, expressing the trehalose-6- phosphate synthase (TPS1) gene of yeast were isolated. In our experimental approach, we applied two novelties, namely the fusion of the drought-inducible promoter StDS2 to TPS1 and a marker-free transformation method. In contrast to the expected drought-induced expression, only a very low constitutive TPS1 expression was detected in the transgenic lines, probably due to chromosomal position effects. The observed expression pattern, however, was sufficient to alter the drought response of plants. Detached leaves of T1 and T2 showed an 8 h delay in wilting compared to the non-transformed control. Potted plants of T1 and T2 kept water 6 days longer than control plants and maintained high stomatal conductance and a satisfactory rate of net photosynthesis. During drought treatment, CO2 assimilation rate measured at saturating CO 2 level was maintained at maximum level for 6-9 days in transgenic plants while it decreased rapidly after 3 days in the wild type plants. Under optimal growth conditions, lower CO2 fixation was detected in the transgenic than in the control plants. Stomatal densities of T1 and T2 leaves were reduced by 30-40%. This may have contributed to the lower CO2 fixation rate and altered drought response.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalPlanta
Volume227
Issue number2
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Droughts
Solanum tuberosum
Saccharomyces cerevisiae
drought
potatoes
water content
Water
Genes
genetically modified organisms
genes
Chromosomal Position Effects
position effect (genetics)
container-grown plants
Genetically Modified Plants
Photosynthesis
wilting
Carbon Monoxide
stomatal conductance
plant response
leaves

Keywords

  • Drought stress
  • Potato
  • Solanaceae
  • Stomata
  • Transgenic plant
  • Trehalose-6-phosphate synthase

ASJC Scopus subject areas

  • Plant Science

Cite this

Effects of drought on water content and photosynthetic parameters in potato plants expressing the trehalose-6-phosphate synthase gene of Saccharomyces cerevisiae. / Stiller, Ibolya; Dulai, Sándor; Kondrák, Mihály; Tarnai, Réka; Szabó, László; Toldi, O.; Bánfalvi, Z.

In: Planta, Vol. 227, No. 2, 01.2008, p. 299-308.

Research output: Contribution to journalArticle

Stiller, Ibolya ; Dulai, Sándor ; Kondrák, Mihály ; Tarnai, Réka ; Szabó, László ; Toldi, O. ; Bánfalvi, Z. / Effects of drought on water content and photosynthetic parameters in potato plants expressing the trehalose-6-phosphate synthase gene of Saccharomyces cerevisiae. In: Planta. 2008 ; Vol. 227, No. 2. pp. 299-308.
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