Effects of different cultivation parameters on the production of surfactin variants by a bacillus subtilis strain

Attila Bartal, Aruna Vigneshwari, Bettina Bóka, Mónika Vörös, István Takács, L. Kredics, L. Manczinger, Mónika Varga, C. Vágvölgyi, A. Szekeres

Research output: Contribution to journalArticle

1 Citation (Scopus)


Surfactins are lipopeptide-Type biosurfactants produced mainly by Bacillus species, consisting of a peptide loop of seven amino acids and a hydrophobic fatty acid chain (C12-C16). These molecules have been proven to exhibit various biological activities; thus, their therapeutic and environmental applications are considered. Within the surfactin lipopeptide family, there is a wide spectrum of different homologues and isomers; to date, more than 30 variants have been described. Since the newest members of these lipopeptides were described recently, there is no information that is available on their characteristic features, e.g., the dependence of their production from different cultivation parameters. This study examined the effects of both the different carbon sources and various metal ions on the surfactin production of a selected B. subtilis strain. Among the applied carbon sources, fructose and xylose had the highest impacts on the ratio of the different variants, regarding both the peptide sequences and the lengths of the fatty acids. Furthermore, the application of metal ions Mn2+, Cu2+ and Ni2+ in the media completely changed the surfactin variant compositions of the fermenting broths leading to the appearance of methyl esterified surfactin forms, and resulted in the appearance of novel surfactin variants with fatty acid chains containing no more than 11 carbon atoms.

Original languageEnglish
Article number2675
Issue number10
Publication statusPublished - Oct 18 2018



  • Bacillus subtilis
  • Cyclic lipopeptides
  • Surfactin production

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this