Effects of deep-freezing and storage time on human femoral cartilage

Balázs Patczai, Tibor Mintál, László Gergely Nőt, Norbert Wiegand, D. Lőrinczy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Surgical techniques including new, possible resources to repair injured joints and damaged cartilage are still evolving. The exact effects of cryopreservation on the collected cartilage samples require accurate determination prior to utilization. The aim of our study was to analyze the impact of cryopreservation at −80 °C on the structural properties of the human cartilage. The effects of storage time were also evaluated in conjunction with optimal utilization. The human cartilage samples were derived during operation and considered to be waste material. Samples were fresh frozen and stored at −80 °C. Cryopreservation times were: 0, 1, 3, 6, and 12 weeks. To assess the biological and structural properties of the frozen human cartilage, we performed calorimetric examinations using differential scanning calorimetry (DSC). During the first 3 weeks, the calorimetric enthalpy (ΔHcal) showed an increasing tendency compared to controls, parallel with the denaturation temperature (Tm): ΔHcal (J g−1) = 1.60 versus 2.49, Tm1 (°C) = 61.73 versus 63.64. After the sixth week, both the enthalpy and the transition temperature decreased, compared to the control samples. The decrease in both the calorimetric enthalpy and Tm could be explained by the decrease in bound water and the time-related degeneration in the structure of the cartilage. Here we found that the duration of cryopreservation interferes with the morphology of human cartilage samples only after 6 weeks of storage time. The thermal analyzes of human cartilage by DSC could be a useful method to follow the morphological changes in the clinical practice.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalJournal of Thermal Analysis and Calorimetry
DOIs
Publication statusAccepted/In press - Mar 10 2016

Fingerprint

cartilage
Cartilage
Freezing
freezing
Enthalpy
enthalpy
Structural properties
Differential scanning calorimetry
heat measurement
Denaturation
scanning
degeneration
biopolymer denaturation
resources
tendencies
Repair
examination
transition temperature
Temperature
temperature

Keywords

  • Cartilage
  • Cryopreservation
  • Femoral neck fracture
  • Mosaic arthroplasty
  • Scanning calorimetry

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics

Cite this

Effects of deep-freezing and storage time on human femoral cartilage. / Patczai, Balázs; Mintál, Tibor; Nőt, László Gergely; Wiegand, Norbert; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, 10.03.2016, p. 1-4.

Research output: Contribution to journalArticle

Patczai, Balázs ; Mintál, Tibor ; Nőt, László Gergely ; Wiegand, Norbert ; Lőrinczy, D. / Effects of deep-freezing and storage time on human femoral cartilage. In: Journal of Thermal Analysis and Calorimetry. 2016 ; pp. 1-4.
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