Effects of decreased specific glutathione reductase activity in a chromate-tolerant mutant of Schizosaccharomyces pombe

Zs Koósz, Z. Gazdag, I. Miklós, Z. Benko, J. Belágyi, J. Antal, B. Meleg, M. Pesti

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Abstract

A chromate-tolerant mutant chr1-663T bearing a stable one-gene mutation and its parental strain 6chr+ were used to investigate the background of CrVI tolerance in the fission yeast Schizosaccharomyces pombe. The mutant chr1-663T displayed a significantly decreased specific glutathione reductase (GR) activity coded by the pgr1 + gene compared with its parental strain. Transformants of the mutant chr1-663T with a nonintegrative pUR18N vector expressing the pgr1 + gene exhibited the same Cr VI sensitivity and specific GR activity as their parental strain, demonstrating the importance of the GR-NADPH system in CrVI tolerance. Transformants, nevertheless, exhibited an increased intracellular peroxide concentration, a decreased CrVI-reducing and HO•-producing ability, which suggested an unbalanced oxidoreduction state of cells and partial complementation of the GR function. No mutation was found in the sequences of the pgr1 + and the pap1 + (transcriptional regulatory gene of GR) genes of the CrVI-tolerant mutant by sequence analysis.

Original languageEnglish
Pages (from-to)308-314
Number of pages7
JournalFolia Microbiologica
Volume53
Issue number4
DOIs
Publication statusPublished - Jul 1 2008

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ASJC Scopus subject areas

  • Microbiology

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