Effects of cytostatics on proliferating and stationary cultures of mammalian cells

E. Oláh, I. Palyi, J. Sugar

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The cytotoxic effects of dibromodulcitol (DBD), dianhydrodulcitol (DAD), vincristine (VCR), N-formyl-leurosine (F-LEU) and bleomycin (BLM) were studied on logarithmically growing and plateau-phase cultures of Chinese hamster cells. One hour treatment with DBD yielded sigmoid type survival curves. Synchronized cells were sensitive to the drug throughout the cell cycle, especially at late G1. DBD killed rapidly growing cells at a higher rate than plateau-phase cells. DAD proved to be equally toxic to both types of cells. At DAD concentrations above 15 μg/ml plateauphase cells were more sensitive than logarithmically growing cultures. The dosedependent survival curves for DAD were of the same type as the DBD curves. VCR and F-LEU produced survival curves characteristic of phase-specific drugs, killing specifically S-phase cells in synchronous cultures. Plateau cells were more sensitive to VCR than log cells, while F-LEU especially killed log cells. BLM produced biphasic survival curves, damaging plateau-phase cells almost 18 times more effectively than log cells.

Original languageEnglish
Pages (from-to)895-900
Number of pages6
JournalEuropean Journal of Cancer (1965)
Volume14
Issue number8
DOIs
Publication statusPublished - 1978

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Cytostatic Agents
Cell Culture Techniques
Dianhydrogalactitol
Mitolactol
Vincristine
Bleomycin
Poisons
Sigmoid Colon
Cricetulus
S Phase
Pharmaceutical Preparations
Cell Cycle

ASJC Scopus subject areas

  • Oncology

Cite this

Effects of cytostatics on proliferating and stationary cultures of mammalian cells. / Oláh, E.; Palyi, I.; Sugar, J.

In: European Journal of Cancer (1965), Vol. 14, No. 8, 1978, p. 895-900.

Research output: Contribution to journalArticle

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