Effects of cytokines on epidermal growth factor receptor expression by malignant trophoblast cells in vitro

M. A. Steller, S. C H Mok, J. Yeh, V. Fülöp, D. J. Anderson, R. S. Berkowitz

Research output: Contribution to journalArticle

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Abstract

Trophoblastic cells abundantly express epidermal growth factor (EGF) receptors, which, when activated by EGF or transforming growth factor-α, can influence cellular growth and metabolism. Various lymphocyte and macrophage cytokines have been found to influence the proliferation of human choriocarcinoma (CCA) cells in vitro. In the current study we investigated the possibility that certain cytokine effects are mediated by changes in EGF receptor expression. JEG-3 human CCA cells were incubated with varying concentrations of interleukin 1-α (IL-1α), interleukin 1-β (IL-1β), interleukin 2, γ-interferon, granulocyte-macrophage colony stimulating factor and tumor necrosis factor-α (TNF), and the expression of EGF receptor was measured by radioimmunoassay using a murine monoclonal antibody with specificity for the EGF receptor. Proliferative or growth suppressive effects of the cytokines were assessed by quantitative analysis of the DNA in the cell culture wells. Macrophage-derived cytokines IL-1α, IL-1β and TNF significantly suppressed cell growth; this was associated with a significant increase in EGF receptor expression. The other cytokines had no significant effect on either EGF receptor expression or cell growth. We also studied the expression of EGF mRNA in JEG-3, Jar and BeWo CCA cell lines. By reverse transcription followed by polymerase chain reaction, low levels of EGF mRNA were detected in all three cell lines. Therefore, EGF may be synthesized by JEG-3, Jar and BeWo CCA cell lines to participate in an autocrine growth pathway. Our findings support the concept that cytokines may act as paracrine mediators of autocrine processes involved in CCA cell growth regulation by modulating growth factor receptor expression.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalJournal of Reproductive Medicine for the Obstetrician and Gynecologist
Volume39
Issue number3
Publication statusPublished - 1994

Fingerprint

Trophoblasts
Choriocarcinoma
Epidermal Growth Factor Receptor
Interleukin-1
Cytokines
Epidermal Growth Factor
Growth
Cell Line
Tumor Necrosis Factor-alpha
Macrophages
Messenger RNA
Antibody Specificity
Growth Factor Receptors
Transforming Growth Factors
Granulocyte-Macrophage Colony-Stimulating Factor
Interferons
Reverse Transcription
Radioimmunoassay
Interleukin-2
In Vitro Techniques

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Effects of cytokines on epidermal growth factor receptor expression by malignant trophoblast cells in vitro. / Steller, M. A.; Mok, S. C H; Yeh, J.; Fülöp, V.; Anderson, D. J.; Berkowitz, R. S.

In: Journal of Reproductive Medicine for the Obstetrician and Gynecologist, Vol. 39, No. 3, 1994, p. 209-216.

Research output: Contribution to journalArticle

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