Effects of cyclosporins and transforming growth factor β1 on thyroid hormone action in cultured fetal rat limb bones

P. Lakatos, Paula H. Stern

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To study the mechanism of action of thyroid hormones on bone, we examined the effects of immunosuppresive and nonimmunosuppressive cyclosporins, as well as of transforming growth factor β1 (TGFβ1), 17β-estradiol (E2), and dihydroxytestosterone (DHT) on thyroxine (T4)-and triiodothyronine (T3)-stimulated bone resorption in fetal rat limb bones. The immunosuppressive cyclosporins A (CsA) and G (CsG) inhibited thyroid hormone (T4+T3)-stimulated resorption and β-glucuronidase release into the culture medium, whereas the weak or nonimmunosuppressive cyclosporins D (CsD) and H (CsH) did not show this effect. Increasing the medium calcium concentration reduced the ability of T4 to stimulate 45Ca release, while not significantly affecting the response to CsA. TGFβ1 elicited a biphasic effect when administered together with T4. During the first 3 days of culture, TGFβ1 elicited a small, nonsignificant decrease in released 45Ca; during a subsequent 3 days of culture, it enhanced T4-stimulated bone resorption significantly. These effects differed from those of TGFβ1 on parathormone-stimulated resorption. E2 and DHT did not influence the action of T4 on bone tissue. These results suggest that the mechanism of action of thyroid hormones on bone may involve immune factors, as well.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalCalcified Tissue International
Volume50
Issue number2
DOIs
Publication statusPublished - Feb 1992

Fingerprint

Cyclosporins
Transforming Growth Factors
Thyroid Hormones
Extremities
Bone and Bones
Triiodothyronine
Bone Resorption
Thyroxine
Cyclosporine
Glucuronidase
Immunologic Factors
Immunosuppressive Agents
Parathyroid Hormone
Culture Media
Estradiol
Calcium

Keywords

  • Bone resorption
  • Cyclosporins
  • TGFβ
  • Thyroid hormones

ASJC Scopus subject areas

  • Endocrinology

Cite this

Effects of cyclosporins and transforming growth factor β1 on thyroid hormone action in cultured fetal rat limb bones. / Lakatos, P.; Stern, Paula H.

In: Calcified Tissue International, Vol. 50, No. 2, 02.1992, p. 123-128.

Research output: Contribution to journalArticle

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