Effects of combined sex hormone replacement therapy on small artery biomechanics in pharmacologically ovariectomized rats

N. Ács, B. Székács, G. Nádasy, S. Várbíró, Zsuzsanna Miklós, M. átyás Szentiványi, E. Monos

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objectives: The purpose of this study was to determine the effects of long-term combined sexual hormone replacement therapy on the biomechanical properties of the small artery wall in castrated female rats. Methods: 30 non-pregnant mature female Sprague-Dawley rats were pharmacologically ovariectomized with 750 μg/kg triptorelin im. every 4th week. Ten of them received combined hormone replacement in form of 15 mg/kg medroxyprogesterone acetate (MPA) im. every 2 weeks and 450 μg/kg estradiol propionate im. once a week. Ten castrated animals received MPA only. Ten control, castrated animals were given the vehicles of these steroids. Ten other animals were kept parallelly, receiving the vehicles of all drugs (control animals). After 12 weeks of treatment cylindrical segments of the saphenous artery were isolated and cannulated at both ends and subjected to in vitro microarteriographic test. Pressure-diameter curves, in the range of 0-200 mmHg, were recorded from segments in normal Krebs-Ringer (nKR) solution, in contraction with norepinephrine (1.6x10-5 M), and then in relaxation with papaverine (2.8x10-5 M). Biomechanical parameters were calculated based on the pressure-diameter curves. Results: Combined hormone replacement therapy significantly increased the passive diameter of small arteries, as compared to those from ovariectomized animals without hormone replacement. MPA monotherapy did not alter the vessel diameter, the inner radii at 100 mmHg intraluminal pressure were, 300±9 μm in the control castrated, 340±7 μm in the estradiol+MPA replaced and 306±8 μm in the MPA treated groups (P

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalMaturitas
Volume34
Issue number1
DOIs
Publication statusPublished - Jan 15 2000

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Medroxyprogesterone Acetate
Biomechanics
Hormone Replacement Therapy
Gonadal Steroid Hormones
Biomechanical Phenomena
Rats
Animals
Arteries
Hormones
Pressure
Estradiol
Triptorelin Pamoate
Papaverine
Drug and Narcotic Control
Propionates
Sprague Dawley Rats
Norepinephrine
Steroids
Pharmaceutical Preparations

Keywords

  • Hormone replacement therapy
  • Medroxyprogesterone
  • Rat
  • Resistance vessel and vascular biomechanics
  • Small artery

ASJC Scopus subject areas

  • Ageing
  • Obstetrics and Gynaecology

Cite this

Effects of combined sex hormone replacement therapy on small artery biomechanics in pharmacologically ovariectomized rats. / Ács, N.; Székács, B.; Nádasy, G.; Várbíró, S.; Miklós, Zsuzsanna; Szentiványi, M. átyás; Monos, E.

In: Maturitas, Vol. 34, No. 1, 15.01.2000, p. 83-92.

Research output: Contribution to journalArticle

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abstract = "Objectives: The purpose of this study was to determine the effects of long-term combined sexual hormone replacement therapy on the biomechanical properties of the small artery wall in castrated female rats. Methods: 30 non-pregnant mature female Sprague-Dawley rats were pharmacologically ovariectomized with 750 μg/kg triptorelin im. every 4th week. Ten of them received combined hormone replacement in form of 15 mg/kg medroxyprogesterone acetate (MPA) im. every 2 weeks and 450 μg/kg estradiol propionate im. once a week. Ten castrated animals received MPA only. Ten control, castrated animals were given the vehicles of these steroids. Ten other animals were kept parallelly, receiving the vehicles of all drugs (control animals). After 12 weeks of treatment cylindrical segments of the saphenous artery were isolated and cannulated at both ends and subjected to in vitro microarteriographic test. Pressure-diameter curves, in the range of 0-200 mmHg, were recorded from segments in normal Krebs-Ringer (nKR) solution, in contraction with norepinephrine (1.6x10-5 M), and then in relaxation with papaverine (2.8x10-5 M). Biomechanical parameters were calculated based on the pressure-diameter curves. Results: Combined hormone replacement therapy significantly increased the passive diameter of small arteries, as compared to those from ovariectomized animals without hormone replacement. MPA monotherapy did not alter the vessel diameter, the inner radii at 100 mmHg intraluminal pressure were, 300±9 μm in the control castrated, 340±7 μm in the estradiol+MPA replaced and 306±8 μm in the MPA treated groups (P",
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AU - Székács, B.

AU - Nádasy, G.

AU - Várbíró, S.

AU - Miklós, Zsuzsanna

AU - Szentiványi, M. átyás

AU - Monos, E.

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