Effects of clary sage oil and its main components, linalool and linalyl acetate, on the plasma membrane of Candida albicans

an in vivo EPR study

Ágnes Blaskó, Zoltán Gazdag, P. Gróf, Gábor Máté, Szilvia Sárosi, Judit Krisch, C. Vágvölgyi, Lilla Makszin, Miklós Pesti

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effects of clary sage (Salvia sclarea L.) oil (CS-oil), and its two main components, linalool (Lol) and linalyl acetate (LA), on cells of the eukaryotic human pathogen yeast Candida albicans were studied. Dynamic and thermodynamic properties of the plasma membrane were investigated by electron paramagnetic resonance (EPR) spectroscopy, with 5-doxylstearic acid (5-SASL) and 16-SASL as spin labels. The monitoring of the head group regions with 5-SASL revealed break-point frequency decrease in a temperature dependent manner of the plasma membrane between 9.55 and 13.15 °C in untreated, in CS-oil-, Lol- and LA-treated membranes. The results suggest a significant increase in fluidity of the treated plasma membranes close to the head groups. Comparison of the results observed with the two spin labels demonstrated that CS-oil and LA induced an increased level of fluidization at both depths of the plasma membrane. Whereas Lol treatment induced a less (1 %) ordered bilayer organization in the superficial regions and an increased (10 %) order of the membrane leaflet in deeper layers. Acute toxicity tests and EPR results indicated that both the apoptotic and the effects exerted on the plasma membrane fluidity depended on the composition and chemical structure of the examined materials. In comparison with the control, treatment with CS-oil, Lol or LA induced 13.0, 12.3 and 26.4 % loss respectively, of the metabolites absorbing at 260 nm, as a biological consequence of the plasma membrane fluidizing effects. Our results confirmed that clary sage oil causes plasma membrane perturbations which leads to cell apoptosis process.

Original languageEnglish
Pages (from-to)175-187
Number of pages13
JournalApoptosis
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 1 2017

Fingerprint

Candida
Electron Spin Resonance Spectroscopy
Cell membranes
Candida albicans
Paramagnetic resonance
Oils
Cell Membrane
Spin Labels
Fluidity
Fluidization
Acute Toxicity Tests
Salvia
Membranes
Membrane Fluidity
Eukaryotic Cells
Pathogens
Metabolites
linalyl acetate
linalool
Thermodynamics

Keywords

  • Candida albicans
  • Clary sage
  • Linalool
  • Linalyl acetate
  • Phase-transition temperature
  • Plasma membrane

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Cancer Research

Cite this

Effects of clary sage oil and its main components, linalool and linalyl acetate, on the plasma membrane of Candida albicans : an in vivo EPR study. / Blaskó, Ágnes; Gazdag, Zoltán; Gróf, P.; Máté, Gábor; Sárosi, Szilvia; Krisch, Judit; Vágvölgyi, C.; Makszin, Lilla; Pesti, Miklós.

In: Apoptosis, Vol. 22, No. 2, 01.02.2017, p. 175-187.

Research output: Contribution to journalArticle

Blaskó, Ágnes ; Gazdag, Zoltán ; Gróf, P. ; Máté, Gábor ; Sárosi, Szilvia ; Krisch, Judit ; Vágvölgyi, C. ; Makszin, Lilla ; Pesti, Miklós. / Effects of clary sage oil and its main components, linalool and linalyl acetate, on the plasma membrane of Candida albicans : an in vivo EPR study. In: Apoptosis. 2017 ; Vol. 22, No. 2. pp. 175-187.
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abstract = "The effects of clary sage (Salvia sclarea L.) oil (CS-oil), and its two main components, linalool (Lol) and linalyl acetate (LA), on cells of the eukaryotic human pathogen yeast Candida albicans were studied. Dynamic and thermodynamic properties of the plasma membrane were investigated by electron paramagnetic resonance (EPR) spectroscopy, with 5-doxylstearic acid (5-SASL) and 16-SASL as spin labels. The monitoring of the head group regions with 5-SASL revealed break-point frequency decrease in a temperature dependent manner of the plasma membrane between 9.55 and 13.15 °C in untreated, in CS-oil-, Lol- and LA-treated membranes. The results suggest a significant increase in fluidity of the treated plasma membranes close to the head groups. Comparison of the results observed with the two spin labels demonstrated that CS-oil and LA induced an increased level of fluidization at both depths of the plasma membrane. Whereas Lol treatment induced a less (1 {\%}) ordered bilayer organization in the superficial regions and an increased (10 {\%}) order of the membrane leaflet in deeper layers. Acute toxicity tests and EPR results indicated that both the apoptotic and the effects exerted on the plasma membrane fluidity depended on the composition and chemical structure of the examined materials. In comparison with the control, treatment with CS-oil, Lol or LA induced 13.0, 12.3 and 26.4 {\%} loss respectively, of the metabolites absorbing at 260 nm, as a biological consequence of the plasma membrane fluidizing effects. Our results confirmed that clary sage oil causes plasma membrane perturbations which leads to cell apoptosis process.",
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AU - Gazdag, Zoltán

AU - Gróf, P.

AU - Máté, Gábor

AU - Sárosi, Szilvia

AU - Krisch, Judit

AU - Vágvölgyi, C.

AU - Makszin, Lilla

AU - Pesti, Miklós

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