Effects of carbodiimides coupling agents on the properties of immobilized glucoamylases

D. Gombin, G. Klamár, J. Krisch, B. Polyák, B. Szajáni

Research output: Contribution to journalArticle

Abstract

Glucoamylase (1,4-α-D-glucan glucohydrolase, EC 3. 2. 1. 3) produced by Aspergillus niger was immobilized on polyacrylamide beads possessing carboxylic functional groups activated by water-soluble carbodiimides bearing different substituents. The most favourable carbodiimide was N-t-butyl-N'-dimethyl-aminopropyl carbodiimide methyl iodide. The structure of the carbodiimide was found to influence the pH and temperature dependence of the catalytic activity, and the substrate specificity of the immobilized enzymes.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalActa Biologica Szegediensis
Volume43
Issue number1-4
Publication statusPublished - 1999

Fingerprint

methyl iodide
Glucan 1,4-alpha-Glucosidase
Carbodiimides
glucan 1,4-alpha-glucosidase
immobilized enzymes
Coupling agents
glucans
polyacrylamide
substrate specificity
hydrolases
Aspergillus niger
catalytic activity
temperature
Bearings (structural)
water
Immobilized Enzymes
Aspergillus
Substrate Specificity
Functional groups
Catalyst activity

Keywords

  • Carbodiimides
  • Glucoamylase
  • Immobilized

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Effects of carbodiimides coupling agents on the properties of immobilized glucoamylases. / Gombin, D.; Klamár, G.; Krisch, J.; Polyák, B.; Szajáni, B.

In: Acta Biologica Szegediensis, Vol. 43, No. 1-4, 1999, p. 69-74.

Research output: Contribution to journalArticle

Gombin, D. ; Klamár, G. ; Krisch, J. ; Polyák, B. ; Szajáni, B. / Effects of carbodiimides coupling agents on the properties of immobilized glucoamylases. In: Acta Biologica Szegediensis. 1999 ; Vol. 43, No. 1-4. pp. 69-74.
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