Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles

B. Sarkadi, A. Schubert, G. Gárdos

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.

Original languageEnglish
Pages (from-to)1045-1047
Number of pages3
JournalExperientia
Volume35
Issue number8
DOIs
Publication statusPublished - Aug 1979

Fingerprint

Active Biological Transport
Egtazic Acid
Cell membranes
Buffers
Cell Membrane
Calcium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles. / Sarkadi, B.; Schubert, A.; Gárdos, G.

In: Experientia, Vol. 35, No. 8, 08.1979, p. 1045-1047.

Research output: Contribution to journalArticle

@article{77a9f1f75ab64d6cb528bd1f3c46e2e0,
title = "Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles",
abstract = "In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.",
author = "B. Sarkadi and A. Schubert and G. G{\'a}rdos",
year = "1979",
month = "8",
doi = "10.1007/BF01949932",
language = "English",
volume = "35",
pages = "1045--1047",
journal = "Cellular and Molecular Life Sciences",
issn = "1420-682X",
publisher = "Birkhauser Verlag Basel",
number = "8",

}

TY - JOUR

T1 - Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles

AU - Sarkadi, B.

AU - Schubert, A.

AU - Gárdos, G.

PY - 1979/8

Y1 - 1979/8

N2 - In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.

AB - In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.

UR - http://www.scopus.com/inward/record.url?scp=0018646248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018646248&partnerID=8YFLogxK

U2 - 10.1007/BF01949932

DO - 10.1007/BF01949932

M3 - Article

C2 - 113247

AN - SCOPUS:0018646248

VL - 35

SP - 1045

EP - 1047

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-682X

IS - 8

ER -