Effects of articaine and ropivacaine on calcium handling and contractility in canine ventricular myocardium

N. Szentandrássy, Adrienn Szabó, János Almássy, I. Jóna, Balázs Horváth, Gergely Szabó, Tamás Bányász, I. Márton, P. Nánási, J. Magyar

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Abstract

Background and objective In spite of the widespread linical use of articaine and ropivacaine there is little information available on the effects of these drugs on myocardial Ca2+ handling. In the present study, therefore, the concentration-dependent effects of articaine and ropivacaine on the components of intracellular Ca2+ handling were studied and compared in canine ventricular myocardium. Methods Contractility was measured in ventricular trabeculae, [Ca2+ ]i transients were recorded from electrically stimulated isolated ventricular myocytes loaded with the calcium-sensitive dye fura-2, L-type Ca2+ current was recorded under whole cell patch clamp conditions, and the release and reuptake of Ca 2+ was monitored in sarcoplasmic reticulum vesicles. Results Articaine and ropivacaine caused a reversible and concentration-dependent decrease in amplitude of the [Ca2+ ]i transient (EC 50=87.4±12 and 99.3±17μmoll-1 , respectively), which was congruent with the reduction obtained for contractility (EC50=73.7±10 and 72.8±14μmoll-1 , respectively). No significant change in diastolic [Ca2+ ]i was found. L-type Ca2+ current was significantly reduced by articaine and ropivacaine with EC50 values of 327±56 and 263±67μmoll-1, respectively. Neither Ca2+ release and Ca2+ uptake nor the ATPase activity of the sarcoplasmic reticulum vesicles was altered by articaine or ropivacaine at concentrations less than 200μmoll-1 . In summary, articaine and ropivacaine caused no significant changes at the therapeutically relevant concentrations of the micromolar range. No significant differences between the effects of articaine and ropivacaine on contractility, [Ca2+ ]i transients, L-type Ca2+ current, and sarcoplasmic reticulum Ca 2+ release and uptake were observed. Conclusions Under conditions of normal application both articaine and ropivacaine are free of cardiodepressant effects; however, a negative inotropic action can be anticipated in cases of accidental intravenous injection or overdose. The observed negative inotropic actions of articaine and ropivacaine are similar in magnitude, and can be mainly attributed to a reduction in net trans-sarcolemmal Ca2+ influx.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalEuropean Journal of Anaesthesiology
Volume27
Issue number2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Carticaine
Canidae
Myocardium
Calcium
Sarcoplasmic Reticulum
ropivacaine
Fura-2
Intravenous Injections
Muscle Cells
Adenosine Triphosphatases

Keywords

  • Articaine
  • Calcium transients
  • Contractility
  • Local anaesthetics
  • Ropivacaine

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Effects of articaine and ropivacaine on calcium handling and contractility in canine ventricular myocardium. / Szentandrássy, N.; Szabó, Adrienn; Almássy, János; Jóna, I.; Horváth, Balázs; Szabó, Gergely; Bányász, Tamás; Márton, I.; Nánási, P.; Magyar, J.

In: European Journal of Anaesthesiology, Vol. 27, No. 2, 02.2010, p. 153-161.

Research output: Contribution to journalArticle

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abstract = "Background and objective In spite of the widespread linical use of articaine and ropivacaine there is little information available on the effects of these drugs on myocardial Ca2+ handling. In the present study, therefore, the concentration-dependent effects of articaine and ropivacaine on the components of intracellular Ca2+ handling were studied and compared in canine ventricular myocardium. Methods Contractility was measured in ventricular trabeculae, [Ca2+ ]i transients were recorded from electrically stimulated isolated ventricular myocytes loaded with the calcium-sensitive dye fura-2, L-type Ca2+ current was recorded under whole cell patch clamp conditions, and the release and reuptake of Ca 2+ was monitored in sarcoplasmic reticulum vesicles. Results Articaine and ropivacaine caused a reversible and concentration-dependent decrease in amplitude of the [Ca2+ ]i transient (EC 50=87.4±12 and 99.3±17μmoll-1 , respectively), which was congruent with the reduction obtained for contractility (EC50=73.7±10 and 72.8±14μmoll-1 , respectively). No significant change in diastolic [Ca2+ ]i was found. L-type Ca2+ current was significantly reduced by articaine and ropivacaine with EC50 values of 327±56 and 263±67μmoll-1, respectively. Neither Ca2+ release and Ca2+ uptake nor the ATPase activity of the sarcoplasmic reticulum vesicles was altered by articaine or ropivacaine at concentrations less than 200μmoll-1 . In summary, articaine and ropivacaine caused no significant changes at the therapeutically relevant concentrations of the micromolar range. No significant differences between the effects of articaine and ropivacaine on contractility, [Ca2+ ]i transients, L-type Ca2+ current, and sarcoplasmic reticulum Ca 2+ release and uptake were observed. Conclusions Under conditions of normal application both articaine and ropivacaine are free of cardiodepressant effects; however, a negative inotropic action can be anticipated in cases of accidental intravenous injection or overdose. The observed negative inotropic actions of articaine and ropivacaine are similar in magnitude, and can be mainly attributed to a reduction in net trans-sarcolemmal Ca2+ influx.",
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T1 - Effects of articaine and ropivacaine on calcium handling and contractility in canine ventricular myocardium

AU - Szentandrássy, N.

AU - Szabó, Adrienn

AU - Almássy, János

AU - Jóna, I.

AU - Horváth, Balázs

AU - Szabó, Gergely

AU - Bányász, Tamás

AU - Márton, I.

AU - Nánási, P.

AU - Magyar, J.

PY - 2010/2

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N2 - Background and objective In spite of the widespread linical use of articaine and ropivacaine there is little information available on the effects of these drugs on myocardial Ca2+ handling. In the present study, therefore, the concentration-dependent effects of articaine and ropivacaine on the components of intracellular Ca2+ handling were studied and compared in canine ventricular myocardium. Methods Contractility was measured in ventricular trabeculae, [Ca2+ ]i transients were recorded from electrically stimulated isolated ventricular myocytes loaded with the calcium-sensitive dye fura-2, L-type Ca2+ current was recorded under whole cell patch clamp conditions, and the release and reuptake of Ca 2+ was monitored in sarcoplasmic reticulum vesicles. Results Articaine and ropivacaine caused a reversible and concentration-dependent decrease in amplitude of the [Ca2+ ]i transient (EC 50=87.4±12 and 99.3±17μmoll-1 , respectively), which was congruent with the reduction obtained for contractility (EC50=73.7±10 and 72.8±14μmoll-1 , respectively). No significant change in diastolic [Ca2+ ]i was found. L-type Ca2+ current was significantly reduced by articaine and ropivacaine with EC50 values of 327±56 and 263±67μmoll-1, respectively. Neither Ca2+ release and Ca2+ uptake nor the ATPase activity of the sarcoplasmic reticulum vesicles was altered by articaine or ropivacaine at concentrations less than 200μmoll-1 . In summary, articaine and ropivacaine caused no significant changes at the therapeutically relevant concentrations of the micromolar range. No significant differences between the effects of articaine and ropivacaine on contractility, [Ca2+ ]i transients, L-type Ca2+ current, and sarcoplasmic reticulum Ca 2+ release and uptake were observed. Conclusions Under conditions of normal application both articaine and ropivacaine are free of cardiodepressant effects; however, a negative inotropic action can be anticipated in cases of accidental intravenous injection or overdose. The observed negative inotropic actions of articaine and ropivacaine are similar in magnitude, and can be mainly attributed to a reduction in net trans-sarcolemmal Ca2+ influx.

AB - Background and objective In spite of the widespread linical use of articaine and ropivacaine there is little information available on the effects of these drugs on myocardial Ca2+ handling. In the present study, therefore, the concentration-dependent effects of articaine and ropivacaine on the components of intracellular Ca2+ handling were studied and compared in canine ventricular myocardium. Methods Contractility was measured in ventricular trabeculae, [Ca2+ ]i transients were recorded from electrically stimulated isolated ventricular myocytes loaded with the calcium-sensitive dye fura-2, L-type Ca2+ current was recorded under whole cell patch clamp conditions, and the release and reuptake of Ca 2+ was monitored in sarcoplasmic reticulum vesicles. Results Articaine and ropivacaine caused a reversible and concentration-dependent decrease in amplitude of the [Ca2+ ]i transient (EC 50=87.4±12 and 99.3±17μmoll-1 , respectively), which was congruent with the reduction obtained for contractility (EC50=73.7±10 and 72.8±14μmoll-1 , respectively). No significant change in diastolic [Ca2+ ]i was found. L-type Ca2+ current was significantly reduced by articaine and ropivacaine with EC50 values of 327±56 and 263±67μmoll-1, respectively. Neither Ca2+ release and Ca2+ uptake nor the ATPase activity of the sarcoplasmic reticulum vesicles was altered by articaine or ropivacaine at concentrations less than 200μmoll-1 . In summary, articaine and ropivacaine caused no significant changes at the therapeutically relevant concentrations of the micromolar range. No significant differences between the effects of articaine and ropivacaine on contractility, [Ca2+ ]i transients, L-type Ca2+ current, and sarcoplasmic reticulum Ca 2+ release and uptake were observed. Conclusions Under conditions of normal application both articaine and ropivacaine are free of cardiodepressant effects; however, a negative inotropic action can be anticipated in cases of accidental intravenous injection or overdose. The observed negative inotropic actions of articaine and ropivacaine are similar in magnitude, and can be mainly attributed to a reduction in net trans-sarcolemmal Ca2+ influx.

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KW - Calcium transients

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KW - Local anaesthetics

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