Effects of an Aminooxy Analogue of Putrescine on Wheat Cell Cultures (Triticum monococcum L.)

G. M. Yakovleva, D. Dudits

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2 Citations (Scopus)

Abstract

The biochemical characterization of a non-morphogenic dedifferentiated cell line of wheat (Triticum monococcum L.) cultured on a medium containing the aminooxy analogue of putrescine, 1-aminooxy-3-aminopropane (APA), revealed altered polyamine metabolism. APA does not inhibit the growth of wheat cells at concentrations up to 5 mM. Exposing the callus tissues to 0.1 mM and 1 mM APA (non-toxic concentrations) results in reduction of activity of both ornithine decarboxylase (ODC) and arginine decarboxylase (ADC) with a simultaneous increase in free putrescine (Put). The contents of cadaverine (Cad) and spermidine (Spd) increase in cultures with 0.1 mM APA as compared with the untreated callus tissues or cultures with 1 mM APA. Neither 0.1 mM APA nor 1 mM APA cause significant changes in spermine (Spm) levels in the treated cells. Under the conditions of severe growth inhibition, APA (5 mM) completely suppresses the ADC activity and decreases the level of free Put in the cell suspension culture.

Original languageEnglish
Pages (from-to)218-221
Number of pages4
JournalJournal of Plant Physiology
Volume142
Issue number2
DOIs
Publication statusPublished - Jan 1 1993

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Keywords

  • 1-Aminooxy-3-aminopropane
  • 1-aminooxy-3-aminopropane
  • ADC
  • APA
  • Cad
  • DFMA
  • DFMO
  • ODC
  • PAs
  • Put
  • Spd
  • Spm
  • Triticum monococcum
  • arginine decarboxylase
  • arginine decarboxylase
  • cadaverine
  • ornithine decarboxylase
  • ornithine decarboxylase
  • polyamines
  • polyamines
  • putrescine
  • spermidine
  • spermine
  • wheat cell cultures
  • α-difluoromethylarginine
  • α-difluoromethylornithine

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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