Effects of amyloid-beta on cholinergic and acetylcholinesterase-positive cells in cultured basal forebrain neurons of embryonic rat brain

P. Kása, Henrietta Papp, Peter Kasa, M. Pákáski, Lajos Balaspiri

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The neurotoxic effects of amyloid-β1-42 and amyloid-β25-35 (Aβ) on cholinergic and acetylcholinesterase-positive neurons were investigated in primary cultures derived from embryonic 18-day-old rat basal forebrain. After various time intervals, the cultures were treated with 1, 5, 10 or 20 μM Aβ for different time periods. The cholinergic neurons and their axon terminals were revealed by vesicular acetylcholine transporter immunohistochemistry and the cholinoceptive cells by acetylcholinesterase histochemical staining. To assess the toxic effects of these Aβ peptides on the cholinergic neurons, image analysis was applied for quantitative determination of the numbers of axon varicosities/terminals and cells. The results demonstrate that, following treatment with 1 or 5 μM Aβ for 5, 10, 30, 60 or 120 min, no changes in vesicular acetylcholine transporter immunohistochemical staining were observed. However, after treatment for 30 min with 10 or 20 μM Aβ, the number of stained axon varicosities was reduced, and treatment for 2 h they had disappeared. In contrast, vesicular acetylcholine transporter-positivity could be seen in some of the neuronal perikarya even after 3 days after treatment. The acetylcholinesterase staining was homogeneously distributed in the control neurons. After Aβ treatment, the histochemical reaction end-product was detected in some of the neuronal perikarya or in the dendritic processes near to the soma. It is concluded that the neurotoxic effects of Aβ appear more rapidly in the cholinergic axon terminals than in the cholinergic and acetylcholinesterase-positive neuronal perikarya.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalBrain Research
Volume998
Issue number1
DOIs
Publication statusPublished - Feb 13 2004

Fingerprint

Acetylcholinesterase
Amyloid
Cholinergic Agents
Vesicular Acetylcholine Transport Proteins
Cultured Cells
Presynaptic Terminals
Neurons
Brain
Cholinergic Neurons
Staining and Labeling
Therapeutics
Poisons
Carisoprodol
Axons
Immunohistochemistry
Basal Forebrain
Peptides

Keywords

  • Acetylcholinesterase
  • Alzheimer's disease
  • Amyloid-β
  • Neuronal culture
  • Neurotoxicity
  • Vesicular acetylcholine transporter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effects of amyloid-beta on cholinergic and acetylcholinesterase-positive cells in cultured basal forebrain neurons of embryonic rat brain. / Kása, P.; Papp, Henrietta; Kasa, Peter; Pákáski, M.; Balaspiri, Lajos.

In: Brain Research, Vol. 998, No. 1, 13.02.2004, p. 73-82.

Research output: Contribution to journalArticle

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