Effects of altered fructose 2,6-bisphosphate levels on carbohydrate metabolism in carnation

Antal Szoke, Erzsébet Kiss, László Heszky, Ildikó Kerepesi, Ottó Toldi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this work was to examine the role of fructose 2,6-bisphosphate (fru 2,6P2) in the carbohydrate metabolism in carnation (Dianthus caryophyllus L.). For this purpose, transgenic plants harboring two modified bifunctional enzyme complementary DNAs of rat liver origin (6-phosphofructo-2- kinase/fructose 2,6-biphosphatase) were generated. Transformation with the kinase construct resulted in a 45% to 85% increase in fru 2,6P2 concentrations compared with the wild type. Transformation with the phosphatase construct reduced the fru 2,6P2 contents by 45% and 70%. These alterations in fru 2,6P2 amounts affected the key enzyme activities of sucrose and starch metabolism. Accordingly, plants with elevated fru 2,6P2 concentrations had high levels of starch, fructose, and triose phosphates, and low levels of sucrose, glucose, and hexose phosphates. In plants with reduced amounts of fru 2,6P2 different results could be observed in major carbohydrate compounds.

Original languageEnglish
Pages (from-to)403-406
Number of pages4
JournalHortScience
Volume42
Issue number2
Publication statusPublished - Apr 1 2007

Keywords

  • Starch
  • Sucrose
  • Transgenic carnation

ASJC Scopus subject areas

  • Horticulture

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