Effects of a structural analogue of salsolinol, 1-MeDIQ, on pituitary prolactin release and dopaminergic activity in the mediobasal hypothalamus in nursing sheep

Tomasz Misztal, Konrad Górski, Dorota Tomaszewska-Zaremba, F. Fülöp, Katarzyna Romanowicz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The prolactin release caused by salsolinol (a derivative of dopamine, DA) in rats could be prevented by its structural analogue 1-methyl-3,4-dihydroisoqinoline (1-MeDIQ). To study the participation of salsolinol in the neural stimulatory mechanism of prolactin release in lactating sheep, we tested whether 1-MeDIQ, acting at the central nervous system (CNS) level, would diminish basal prolactin release and reduce prolactin surge induced by suckling. A series of intracerebroventricular (icv) infusions of 1-MeDIQ (5 × 60 μg/60 μl/30 min, at 30-min intervals) were performed in nursing ewes (n = 8) during the fifth week of lactation. Additionally, by combining these infusions with push-pull perfusion, we studied the concentration of dopaminergic components, i.e., salsolinol, DA and 3,4-dihydroxyphenylacetic acid (DOPAC) within the infundibular nucleus/median eminence (IN/ME) in four of the ewes. Treatment with 1-MeDIQ significantly (P <0.001) reduced either the basal prolactin release during the non-suckling period or the suckling-induced prolactin surge. Specifically, the suppressive effect occurred gradually, affecting both the duration and amplitude of the prolactin surge. In the control ewes, the perfusate salsolinol concentration increased significantly (P <0.001) during suckling, while in the ewes treated with 1-MeDIQ only vestigial amounts of this compound were found during the non-suckling period. No DA was detected in the perfusates collected from the IN/ME of control and 1-MeDIQ-treated groups and no significant differences were found in the DOPAC concentrations between these groups. In conclusion, 1-MeDIQ is able to inhibit prolactin secretion in lactating sheep, acting at the CNS level. In addition, one of the way of 1-MeDIQ action may be directed to the local salsolinol release within the mediobasal hypothalamus.

Original languageEnglish
Pages (from-to)72-77
Number of pages6
JournalBrain Research
Volume1307
DOIs
Publication statusPublished - Jan 11 2010

Fingerprint

Prolactin
Hypothalamus
Sheep
Nursing
Dopamine
Median Eminence
Arcuate Nucleus of Hypothalamus
Central Nervous System
Intraventricular Infusions
3,4-Dihydroxyphenylacetic Acid
salsolinol
Lactation
Perfusion
Acids

Keywords

  • 1-MeDIQ
  • Dopaminergic system
  • lactation
  • Nursing sheep
  • Prolactin release
  • Salsolinol

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Effects of a structural analogue of salsolinol, 1-MeDIQ, on pituitary prolactin release and dopaminergic activity in the mediobasal hypothalamus in nursing sheep. / Misztal, Tomasz; Górski, Konrad; Tomaszewska-Zaremba, Dorota; Fülöp, F.; Romanowicz, Katarzyna.

In: Brain Research, Vol. 1307, 11.01.2010, p. 72-77.

Research output: Contribution to journalArticle

Misztal, Tomasz ; Górski, Konrad ; Tomaszewska-Zaremba, Dorota ; Fülöp, F. ; Romanowicz, Katarzyna. / Effects of a structural analogue of salsolinol, 1-MeDIQ, on pituitary prolactin release and dopaminergic activity in the mediobasal hypothalamus in nursing sheep. In: Brain Research. 2010 ; Vol. 1307. pp. 72-77.
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