Effects of a standardized mistletoe preparation on metastatic B16 melanoma colonization in murine lungs

Klaus Weber, Ulrich Mengs, Tobias Schwarz, Tibor Hajto, Katarina Hostanska, Thomas R. Allen, Roland Weyhenmeyer, Hans Lentzen

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22 Citations (Scopus)

Abstract

The immune response-modifying drug Lektinol® is a mistletoe preparation which is standardized with respect to bioactive viscum album agglutinin, the most active component of mistletoe. The present study was designed to evaluate the antimetastatic effects of this preparation following intravenous injection of B16 melanoma cells into mice. The standardized mistletoe extract was administered intravenously in doses of 100, 1000 or 5000 μl/kg (equivalent to 3, 30 or 150 ng/kg of riscurn album agglutinin) once daily for three weeks. An inhibition of mean pulmonary metastatic colonization of 58 to 95%, as measured by the number of melanoma cells on lung tissue slides, and a significant decrease of percentage of bronchoalveolar lavage pigmented cells were observed. In addition, a correlation of this antirectastatic activity with cellular immune parameters was investigated. In lavage fluids from the tumor-bearing mice, there was a 5 to 6-fold significant increase in the percentage of MAC-1+ (CD11b/CD18) immunocompetent macrophages in comparison with cells from vehicle-treated animals. The percentages of double-positive immature CD4+8+ thymocytes were significantly increased in animals treated with the standardized mistletoe extract. There were no signs of treatment- related toxicity. The results of this study indicate that the standardized mistletoe extract shows antimetastatic activity against B16 melanoma lung colonization.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalArzneimittel-Forschung/Drug Research
Volume48
Issue number5
Publication statusPublished - Jun 10 1998

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Keywords

  • B16 melanoma
  • Cancer immunotherapy
  • Lektinol®
  • Metastasis
  • Mistletoe extract
  • Mouse
  • Phytotherapy
  • Viscum album agglutinin

ASJC Scopus subject areas

  • Drug Discovery

Cite this

Weber, K., Mengs, U., Schwarz, T., Hajto, T., Hostanska, K., Allen, T. R., Weyhenmeyer, R., & Lentzen, H. (1998). Effects of a standardized mistletoe preparation on metastatic B16 melanoma colonization in murine lungs. Arzneimittel-Forschung/Drug Research, 48(5), 497-502.