Effective and reproducible protocols for in vitro culturing and plant regeneration of the physiological model plant Ramonda myconi (L.) Rchb

Sándor Tóth, Peter Scott, Seppo Sorvari, O. Toldi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The first procedures have been established for in vitro micropropagation and plant regeneration of the desiccation-tolerant plant Ramonda myconi (L.) Rchb. An integrated optimisation of salt, antioxidant and growth regulator composition, and potassium-phosphate buffer-mediated pH stabilisation of culture media resulted in an effective tissue culture system that prevented the undesirable hyperhydricity and tissue necrosis typical of Ramonda tissue cultures. We examined whether there is any similarity in tissue culturing between the two resurrection plants Craterostigma plantagineum and R. myconi as a consequence of their common physiological specialisation. The fact that regenerated plants developed on dedifferentiated cell cultures, which is a precondition of Agrobacterium-mediated gene transfer, could mean an applicable basis for the optimisation of the genetic transformation of Ramonda species.

Original languageEnglish
Pages (from-to)1027-1034
Number of pages8
JournalPlant Science
Volume166
Issue number4
DOIs
Publication statusPublished - Apr 2004

Fingerprint

Physiological models
Tissue culture
Craterostigma
Regeneration
Tissue
Gene transfer
tissue culture
Cell culture
Culture Media
Buffers
Stabilization
Salts
Antioxidants
hyperhydricity
Genetic Transformation
potassium phosphates
Desiccation
Agrobacterium
genetic transformation
Chemical analysis

Keywords

  • 6-benzyladenine
  • BAP
  • CMS medium
  • Craterostigma medium [Plant Cell Rep. 21 (2002) 63]
  • Germination medium [Plant Sci. 97 (1994) 217]
  • GM medium
  • MS medium
  • Murashige and Skoog medium (1962)
  • NAA
  • RA medium
  • Ramonda medium (described here)

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Effective and reproducible protocols for in vitro culturing and plant regeneration of the physiological model plant Ramonda myconi (L.) Rchb. / Tóth, Sándor; Scott, Peter; Sorvari, Seppo; Toldi, O.

In: Plant Science, Vol. 166, No. 4, 04.2004, p. 1027-1034.

Research output: Contribution to journalArticle

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