Effect of vitrification on mitochondrial distribution and membrane potential in mouse two pronuclear (2-PN) embryos

Xue Ming Zhao, Xiang Wei Fu, Yun Peng Hou, Chang Liang Yan, Lun Suo, Yan Ping Wang, Hua Bin Zhu, A. Dinnyés, Shi En Zhu

Research output: Contribution to journalArticle

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Abstract

The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Δψ) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial distribution was examined by fluorescence microscopy technology. Results indicated that: (1) The rate of mitochondrial ring formation around pronuclei in vitrified 2-PN embryos was significantly lower than in fresh ones (67.3 ± 3.0% vs. 84.9 ± 3.1%) (P <0.05). (2) Blastocyst development rate of vitrified 2-PN embryos without mitochondrial rings (61.7 ± 4.5%) was significantly lower than that of vitrified embryos with mitochondrial rings (82.1 ± 2.8%). (3) Following staining by 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbo- cyanine iodide (JC-1), most red-colored mitochondria (high Δψ) were distributed peripherally around pronuclei and along cell membranes of fresh 2-PN embryos. Conversely, red-colored mitochondria were greatly diminished in vitrified embryos, with green mitochondria (low Δψ) evenly distributed throughout the cytoplasm. The proportion of fresh 2-PN embryos with obvious aggregation of high Δψ mitochondria (84.2 ± 2.2%) was significantly higher than that of vitrified embryos (26.7 ± 3.0%) (P <0.05). (4) The proportion of fresh embryos with microtubules distributed around pronuclei (83.5 ± 3.4%) was similar to that of vitrified embryos (74.7 ± 2.5%). In conclusion, vitrification affected mitochondrial distribution and decreased the mitochondrial membrane potential in mouse 2-PN embryos, events which may affect subsequent developmental viability of such embryos.

Original languageEnglish
Pages (from-to)1056-1063
Number of pages8
JournalMolecular Reproduction and Development
Volume76
Issue number11
DOIs
Publication statusPublished - Nov 2009

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Vitrification
Mitochondrial Membrane Potential
Embryonic Structures
Mitochondria
Microtubules
Iodides
Blastocyst
Fluorescence Microscopy

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Effect of vitrification on mitochondrial distribution and membrane potential in mouse two pronuclear (2-PN) embryos. / Zhao, Xue Ming; Fu, Xiang Wei; Hou, Yun Peng; Yan, Chang Liang; Suo, Lun; Wang, Yan Ping; Zhu, Hua Bin; Dinnyés, A.; Zhu, Shi En.

In: Molecular Reproduction and Development, Vol. 76, No. 11, 11.2009, p. 1056-1063.

Research output: Contribution to journalArticle

Zhao, Xue Ming ; Fu, Xiang Wei ; Hou, Yun Peng ; Yan, Chang Liang ; Suo, Lun ; Wang, Yan Ping ; Zhu, Hua Bin ; Dinnyés, A. ; Zhu, Shi En. / Effect of vitrification on mitochondrial distribution and membrane potential in mouse two pronuclear (2-PN) embryos. In: Molecular Reproduction and Development. 2009 ; Vol. 76, No. 11. pp. 1056-1063.
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abstract = "The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Δψ) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial distribution was examined by fluorescence microscopy technology. Results indicated that: (1) The rate of mitochondrial ring formation around pronuclei in vitrified 2-PN embryos was significantly lower than in fresh ones (67.3 ± 3.0{\%} vs. 84.9 ± 3.1{\%}) (P <0.05). (2) Blastocyst development rate of vitrified 2-PN embryos without mitochondrial rings (61.7 ± 4.5{\%}) was significantly lower than that of vitrified embryos with mitochondrial rings (82.1 ± 2.8{\%}). (3) Following staining by 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbo- cyanine iodide (JC-1), most red-colored mitochondria (high Δψ) were distributed peripherally around pronuclei and along cell membranes of fresh 2-PN embryos. Conversely, red-colored mitochondria were greatly diminished in vitrified embryos, with green mitochondria (low Δψ) evenly distributed throughout the cytoplasm. The proportion of fresh 2-PN embryos with obvious aggregation of high Δψ mitochondria (84.2 ± 2.2{\%}) was significantly higher than that of vitrified embryos (26.7 ± 3.0{\%}) (P <0.05). (4) The proportion of fresh embryos with microtubules distributed around pronuclei (83.5 ± 3.4{\%}) was similar to that of vitrified embryos (74.7 ± 2.5{\%}). In conclusion, vitrification affected mitochondrial distribution and decreased the mitochondrial membrane potential in mouse 2-PN embryos, events which may affect subsequent developmental viability of such embryos.",
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AU - Zhu, Hua Bin

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