Effect of vitamin e on reperfusion injuries during reconstructive vascular operations on lower limbs

E. Arató, M. Kürthy, L. Sínay, G. Kasza, G. Menyhei, P. Hardi, S. Masoud, K. Ripp, K. Szilágyi, I. Takács, Z. Miklós, A. Bátor, J. Lantos, L. Kollár, E. Rőth, G. Jancsó

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Introduction: The challenge against reperfusion injury and tissue oxidative stress, especially in vascular surgical interventions has an essential importance to reach the optimal clinical result. Numerous experimental attempts have proved the positive antioxidant effect of vitamin E in both chronic and acute phase models. In our study we monitored the effect of continuous preoperative treatment with vitamin E, on oxidative stress and tissue inflammation reactions developed after reconstructive operations. Patients and methods: 32 patients have been involved in a randomized, prospective study, all suffering from AFS occlusion proved by angiography, and all undergone supragenual reconstruction. Duration of ischemia and amount of tissues under vascular clamping were almost the same in all patients. In the group treated with E-vitamin, we administered 1×200 mg of vitamin E p/o from the preoperative day till the 7th post operative day. Patients of the second group did not receive vitamin E. Materials and methods: Peripheral blood samples were collected immediately before operation and at the end of the second reperfusion hour (early reperfusion period). Late reperfusion period has been monitored by analyzing blood samples taken at 24th hour and 7th day next to the operative ischemia. Among oxidative stress parameters, direct measurement of reactive oxygen intermediator (ROI) and determination of antioxidant state (GSH, Total-SH group, SOD) have been performed. Malondialdehyde was chosen as marker for lipidperoxidation. Inflammation reactions were monitored up on expression of adhesion molecules (CD11a and CD18). We also controlled the oscillation of myeloperoxidase (MPO) activity. Results: Our study has proved that preoperative (from the preoperative day till the 7th post operative day) administration of 200 mg vitamin E could reduce the level of oxidative stress developed after ischemic-reperfusion insult (lipidproxidation, antioxidant enzymes). According to our results, the prooxidant-antioxidant imbalance also diminished in the group with E-vitamin treatment. We proved that elective administration of vitamin E could decrease the WBC activity (MPO activity, free radicals production, expression of adhesion molecules) and its consequential local inflammation process, during early reperfusion.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalClinical Hemorheology and Microcirculation
Volume44
Issue number2
DOIs
Publication statusPublished - 2010

Fingerprint

Reperfusion Injury
Vitamin E
Vitamins
Blood Vessels
Lower Extremity
Reperfusion
Oxidative Stress
Antioxidants
Inflammation
Peroxidase
Ischemia
Malondialdehyde
Constriction
Free Radicals
Angiography
Prospective Studies
Oxygen
Enzymes
Therapeutics

Keywords

  • Leukocyte activity
  • Oxidative stress
  • PAD
  • Reperfusion injury
  • Vitamin E

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Effect of vitamin e on reperfusion injuries during reconstructive vascular operations on lower limbs. / Arató, E.; Kürthy, M.; Sínay, L.; Kasza, G.; Menyhei, G.; Hardi, P.; Masoud, S.; Ripp, K.; Szilágyi, K.; Takács, I.; Miklós, Z.; Bátor, A.; Lantos, J.; Kollár, L.; Rőth, E.; Jancsó, G.

In: Clinical Hemorheology and Microcirculation, Vol. 44, No. 2, 2010, p. 125-136.

Research output: Contribution to journalArticle

Arató, E, Kürthy, M, Sínay, L, Kasza, G, Menyhei, G, Hardi, P, Masoud, S, Ripp, K, Szilágyi, K, Takács, I, Miklós, Z, Bátor, A, Lantos, J, Kollár, L, Rőth, E & Jancsó, G 2010, 'Effect of vitamin e on reperfusion injuries during reconstructive vascular operations on lower limbs', Clinical Hemorheology and Microcirculation, vol. 44, no. 2, pp. 125-136. https://doi.org/10.3233/CH-2010-1260
Arató, E. ; Kürthy, M. ; Sínay, L. ; Kasza, G. ; Menyhei, G. ; Hardi, P. ; Masoud, S. ; Ripp, K. ; Szilágyi, K. ; Takács, I. ; Miklós, Z. ; Bátor, A. ; Lantos, J. ; Kollár, L. ; Rőth, E. ; Jancsó, G. / Effect of vitamin e on reperfusion injuries during reconstructive vascular operations on lower limbs. In: Clinical Hemorheology and Microcirculation. 2010 ; Vol. 44, No. 2. pp. 125-136.
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AU - Kürthy, M.

AU - Sínay, L.

AU - Kasza, G.

AU - Menyhei, G.

AU - Hardi, P.

AU - Masoud, S.

AU - Ripp, K.

AU - Szilágyi, K.

AU - Takács, I.

AU - Miklós, Z.

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AU - Lantos, J.

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AU - Rőth, E.

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N2 - Introduction: The challenge against reperfusion injury and tissue oxidative stress, especially in vascular surgical interventions has an essential importance to reach the optimal clinical result. Numerous experimental attempts have proved the positive antioxidant effect of vitamin E in both chronic and acute phase models. In our study we monitored the effect of continuous preoperative treatment with vitamin E, on oxidative stress and tissue inflammation reactions developed after reconstructive operations. Patients and methods: 32 patients have been involved in a randomized, prospective study, all suffering from AFS occlusion proved by angiography, and all undergone supragenual reconstruction. Duration of ischemia and amount of tissues under vascular clamping were almost the same in all patients. In the group treated with E-vitamin, we administered 1×200 mg of vitamin E p/o from the preoperative day till the 7th post operative day. Patients of the second group did not receive vitamin E. Materials and methods: Peripheral blood samples were collected immediately before operation and at the end of the second reperfusion hour (early reperfusion period). Late reperfusion period has been monitored by analyzing blood samples taken at 24th hour and 7th day next to the operative ischemia. Among oxidative stress parameters, direct measurement of reactive oxygen intermediator (ROI) and determination of antioxidant state (GSH, Total-SH group, SOD) have been performed. Malondialdehyde was chosen as marker for lipidperoxidation. Inflammation reactions were monitored up on expression of adhesion molecules (CD11a and CD18). We also controlled the oscillation of myeloperoxidase (MPO) activity. Results: Our study has proved that preoperative (from the preoperative day till the 7th post operative day) administration of 200 mg vitamin E could reduce the level of oxidative stress developed after ischemic-reperfusion insult (lipidproxidation, antioxidant enzymes). According to our results, the prooxidant-antioxidant imbalance also diminished in the group with E-vitamin treatment. We proved that elective administration of vitamin E could decrease the WBC activity (MPO activity, free radicals production, expression of adhesion molecules) and its consequential local inflammation process, during early reperfusion.

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