Effect of viscosity on enzyme-ligand dissociation II. Role of the microenvironment

G. Rickey Welch, Béla Somogyi, János Matkó, Sándor Papp

Research output: Contribution to journalArticle

10 Citations (Scopus)


A theoretical treatment, describing a novel viscosity effect on decomposition of enzyme-ligand complexes, recently appeared (Somogyi et al., 1978). From this approach emerged a mechanistic picture of the manner in which increased viscosity lowers the value of the decomposition rate constant. A refined version of this model is presented herein. The analysis is extended to the molecular microenvironment ultimately responsible for mediating the "viscosity effect". Consideration is given to two major factors: (1) the role of viscosity in attenuating the excess chemical energy and (2) the statistical features of the microviscosity. In view of spatiotemporal inhomogeneity in the liquid structure, the concept of averaged microviscosity is introduced to parametrize the enzyme-ligand recombination probability. Quantitative predictions are consistent with models of liquid structure and with results from enzyme studies. The "viscosity effect" may contribute to substrate compartmentation in organized multienzyme systems in vivo.

Original languageEnglish
Pages (from-to)211-238
Number of pages28
JournalJournal of Theoretical Biology
Issue number2
Publication statusPublished - Jan 21 1983

ASJC Scopus subject areas

  • Statistics and Probability
  • Modelling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Fingerprint Dive into the research topics of 'Effect of viscosity on enzyme-ligand dissociation II. Role of the microenvironment'. Together they form a unique fingerprint.

  • Cite this