Effect of vasoactive peptides on Tetrahymena. Chemotactic properties of endothelins (ET-1, ET-2, ET-3, fragment 11-21 of ET-1 and big endothelin-1): A short-term inducible signalling mechanism of chemotaxis

László Kohidai, Katalin Tóth, Heikki Ruskoaho, György Csaba

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The unicellular Tetrahymena is a sensitive model for the study of chemotaxis induced by endothelins. In short-term chemotactic responses, ET-2 and ET-3 were chemorepellent, compared to the referent control (culture medium) and chemoattractant, ET-1. These differences suggest that the change of some aromatic residues in the loop region (residues 5-9) of the ET-1 abrogates its chemoattractant character. The response of Tetrahymena is highly selective, since substitution of two amino acids are enough to cause this alteration in (behavioural) response. Such a change seems to be more important than the loss of the entire first 10 amino acids (in ET-1 fragment 11-21), since after this it acquires some chemoattractive effect of ET-1. Selection with ET-3 rigorously stimulated the cell's responsiveness to the medium, this ability was abolished by the repeated ET-3 treatment. Big endothelin-1 was repellent in all concentrations. These experiments demonstrate the very sensitive discriminating capacity of chemotactic responsiveness at a low level of phylogeny. Chemotactic selection with endothelins underlines the possibility that there may be separate mechanisms responsible for the short-term chemotactic responses and the long-lasting effects of chemotactic selection.

Original languageEnglish
Pages (from-to)1173-1177
Number of pages5
JournalCell biology international
Volume25
Issue number11
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Chemoattraction
  • Endothelins
  • Hormones
  • Phylogeny
  • Selection
  • Tetrahymena

ASJC Scopus subject areas

  • Cell Biology

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