Effect of vasoactive peptides in Tetrahymena: chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP)

L. Kőhidai, Katalin Tóth, Paul Samotik, Kiran Ranganathan, Orsolya Láng, Miklós Tóth, Heikki Ruskoaho

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Adrenomedullin (AMD), proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP) were studied for chemotaxis, chemotactic selection and G-actin/F-actin transition in Tetrahymena. The aim of the experiments was to study the effects of two different peptides encoded by the same gene compared to a peptide related to one of the two, but encoded by a different gene, at a low level of phylogeny. The positive, chemotactic effect of ADM and the strong negative, chemorepellent effect of PAMP suggest that in Tetrahymena, the two peptides elicit their chemotactic effects via different signalling mechanisms. The complexity of swimming behaviour modulated by the three peptides underlines that chemotaxis, chemokinesis and some characteristics of migratory behaviour (velocity, tortuosity) are working as a sub-population level complex functional unit. Chemotactic responsiveness to ADM and CGRP is short-term, in contrast to PAMP, which as a chemorepellent ligand, has the ability to select sub-populations with negative chemotactic responsiveness. The different effects of ADM and PAMP on the polymerization of actin networks show that the microtubular structure of cilia is more essential to chemotactic response than are transitions of the actin network. The results draw attention to the characteristic effects of vasoactive peptides at this low level of phylogeny.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume411
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Adrenomedullin
Tetrahymena
Calcitonin Gene-Related Peptide
Delta modulation
Actins
Peptides
Chemotaxis
Phylogeny
Genes
Aptitude
Cilia
Polymerization
Population
Pathogen-Associated Molecular Pattern Molecules
Ligands
Experiments

Keywords

  • Adrenomedullin
  • CGRP
  • Migration
  • PAMP
  • Tetrahymena

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Effect of vasoactive peptides in Tetrahymena : chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP). / Kőhidai, L.; Tóth, Katalin; Samotik, Paul; Ranganathan, Kiran; Láng, Orsolya; Tóth, Miklós; Ruskoaho, Heikki.

In: Molecular and Cellular Biochemistry, Vol. 411, No. 1-2, 01.01.2016, p. 271-280.

Research output: Contribution to journalArticle

Kőhidai, L. ; Tóth, Katalin ; Samotik, Paul ; Ranganathan, Kiran ; Láng, Orsolya ; Tóth, Miklós ; Ruskoaho, Heikki. / Effect of vasoactive peptides in Tetrahymena : chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP). In: Molecular and Cellular Biochemistry. 2016 ; Vol. 411, No. 1-2. pp. 271-280.
@article{b7be90cf61bd43b7bd0c3d080d4d4f10,
title = "Effect of vasoactive peptides in Tetrahymena: chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP)",
abstract = "Adrenomedullin (AMD), proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP) were studied for chemotaxis, chemotactic selection and G-actin/F-actin transition in Tetrahymena. The aim of the experiments was to study the effects of two different peptides encoded by the same gene compared to a peptide related to one of the two, but encoded by a different gene, at a low level of phylogeny. The positive, chemotactic effect of ADM and the strong negative, chemorepellent effect of PAMP suggest that in Tetrahymena, the two peptides elicit their chemotactic effects via different signalling mechanisms. The complexity of swimming behaviour modulated by the three peptides underlines that chemotaxis, chemokinesis and some characteristics of migratory behaviour (velocity, tortuosity) are working as a sub-population level complex functional unit. Chemotactic responsiveness to ADM and CGRP is short-term, in contrast to PAMP, which as a chemorepellent ligand, has the ability to select sub-populations with negative chemotactic responsiveness. The different effects of ADM and PAMP on the polymerization of actin networks show that the microtubular structure of cilia is more essential to chemotactic response than are transitions of the actin network. The results draw attention to the characteristic effects of vasoactive peptides at this low level of phylogeny.",
keywords = "Adrenomedullin, CGRP, Migration, PAMP, Tetrahymena",
author = "L. Kőhidai and Katalin T{\'o}th and Paul Samotik and Kiran Ranganathan and Orsolya L{\'a}ng and Mikl{\'o}s T{\'o}th and Heikki Ruskoaho",
year = "2016",
month = "1",
day = "1",
doi = "10.1007/s11010-015-2589-6",
language = "English",
volume = "411",
pages = "271--280",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Effect of vasoactive peptides in Tetrahymena

T2 - chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP)

AU - Kőhidai, L.

AU - Tóth, Katalin

AU - Samotik, Paul

AU - Ranganathan, Kiran

AU - Láng, Orsolya

AU - Tóth, Miklós

AU - Ruskoaho, Heikki

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Adrenomedullin (AMD), proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP) were studied for chemotaxis, chemotactic selection and G-actin/F-actin transition in Tetrahymena. The aim of the experiments was to study the effects of two different peptides encoded by the same gene compared to a peptide related to one of the two, but encoded by a different gene, at a low level of phylogeny. The positive, chemotactic effect of ADM and the strong negative, chemorepellent effect of PAMP suggest that in Tetrahymena, the two peptides elicit their chemotactic effects via different signalling mechanisms. The complexity of swimming behaviour modulated by the three peptides underlines that chemotaxis, chemokinesis and some characteristics of migratory behaviour (velocity, tortuosity) are working as a sub-population level complex functional unit. Chemotactic responsiveness to ADM and CGRP is short-term, in contrast to PAMP, which as a chemorepellent ligand, has the ability to select sub-populations with negative chemotactic responsiveness. The different effects of ADM and PAMP on the polymerization of actin networks show that the microtubular structure of cilia is more essential to chemotactic response than are transitions of the actin network. The results draw attention to the characteristic effects of vasoactive peptides at this low level of phylogeny.

AB - Adrenomedullin (AMD), proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP) were studied for chemotaxis, chemotactic selection and G-actin/F-actin transition in Tetrahymena. The aim of the experiments was to study the effects of two different peptides encoded by the same gene compared to a peptide related to one of the two, but encoded by a different gene, at a low level of phylogeny. The positive, chemotactic effect of ADM and the strong negative, chemorepellent effect of PAMP suggest that in Tetrahymena, the two peptides elicit their chemotactic effects via different signalling mechanisms. The complexity of swimming behaviour modulated by the three peptides underlines that chemotaxis, chemokinesis and some characteristics of migratory behaviour (velocity, tortuosity) are working as a sub-population level complex functional unit. Chemotactic responsiveness to ADM and CGRP is short-term, in contrast to PAMP, which as a chemorepellent ligand, has the ability to select sub-populations with negative chemotactic responsiveness. The different effects of ADM and PAMP on the polymerization of actin networks show that the microtubular structure of cilia is more essential to chemotactic response than are transitions of the actin network. The results draw attention to the characteristic effects of vasoactive peptides at this low level of phylogeny.

KW - Adrenomedullin

KW - CGRP

KW - Migration

KW - PAMP

KW - Tetrahymena

UR - http://www.scopus.com/inward/record.url?scp=84957441204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957441204&partnerID=8YFLogxK

U2 - 10.1007/s11010-015-2589-6

DO - 10.1007/s11010-015-2589-6

M3 - Article

C2 - 26481478

AN - SCOPUS:84957441204

VL - 411

SP - 271

EP - 280

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -