Effect of Val34Leu polymorphism on the activation of the coagulation factor XIII-A

U. Wartiovaara, H. Mikkola, G. Szoke, G. Haramura, L. Karpati, I. Balogh, R. Lassila, L. Muszbek, A. Palotie

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Coagulation factor XIII (FXIII) is a protransglutaminase involved in the last step of the coagulation cascade by stabilising the fibrin clot. Recently, a common variation (FXIII Val34Leu) has been associated with a decreased risk of myocardial infarction and deep venous thrombosis. Val34Leu is critically located near the thrombin activation site of FXIII-A. In this study we investigated its effects on the activation of FXIII. Both recombinant and platelet-derived FXIII Val34Leu variants were shown to be more susceptible to thrombin cleavage than the wild type FXIII. The rate of enzymatic activation of FXIII Val34Leu was found increased, however, the specific activity of fully activated wild type FXIII and the Val34Leu mutant did not differ. During the course of thrombin-induced activation of FXIII fibrin γ-chain dimerisation and α-chain polymerisation developed more rapidly with the Val34Leu mutant. The increased rate of fibrin stabilisation brought about by the Val34Leu FXIII seems to be paradoxically associated with a protective effect against pathological thrombosis.

Original languageEnglish
Pages (from-to)595-600
Number of pages6
JournalThrombosis and Haemostasis
Volume84
Issue number4
Publication statusPublished - Jan 1 2000

    Fingerprint

Keywords

  • Activation peptide
  • Factor XIII
  • Factor XIII Val34Leu
  • Factor XIII activation
  • Thrombin

ASJC Scopus subject areas

  • Hematology

Cite this