Effect of tumor-conditioned medium on intercellular communication and proliferation of Balb/c 3T3 cells

L. Tóth, Gabriella Pásti, Attila Sárváry, M. Balázs, R. Ádány

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The possible role of tumor cell-derived factors in the regulation of gap junctional intercellular communication and proliferation of fibroblasts was studied in a model system of Balb/c 3T3 cells growing in tumor conditioned medium by Lucifer Yellow CH dye-transfer and BrdU incorporation assays. Six to 24 h incubation of Balb/c 3T3 cells in a medium conditioned by WiDr adenocarcinoma cells enhanced the gap junctional communication between the cells by 25-40% as revealed by intercellular transfer of a fluorescent dye Lucifer Yellow CH. Simultaneously the cell proliferation rates were examined and found to be reduced by 23% at 24 h treatment. Since adenocarcinoma cells are known to secrete different growth factor-like polypeptides into their conditioned medium, we suppose that tumors that produce these molecules might alter their host environment through the enhancement of cell-cell communication thereby facilitating the exchange of modulatory factors. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalCancer Letters
Volume151
Issue number1
DOIs
Publication statusPublished - Apr 3 2000

Fingerprint

3T3 Cells
Conditioned Culture Medium
Cell Communication
Neoplasms
Adenocarcinoma
Bromodeoxyuridine
Fluorescent Dyes
Intercellular Signaling Peptides and Proteins
Coloring Agents
Fibroblasts
Cell Proliferation
Peptides
lucifer yellow

Keywords

  • Balb/c 3T3 cells
  • Cell proliferation
  • Intercellular communication
  • Tumor-conditioned medium
  • WiDr cells

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Effect of tumor-conditioned medium on intercellular communication and proliferation of Balb/c 3T3 cells. / Tóth, L.; Pásti, Gabriella; Sárváry, Attila; Balázs, M.; Ádány, R.

In: Cancer Letters, Vol. 151, No. 1, 03.04.2000, p. 57-61.

Research output: Contribution to journalArticle

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