Effect of tumor-conditioned medium on intercellular communication and proliferation of Balb/c 3T3 cells

László Tóth, Gabriella Pásti, Attila Sárváry, Margit Balázs, Róza Ádány

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The possible role of tumor cell-derived factors in the regulation of gap junctional intercellular communication and proliferation of fibroblasts was studied in a model system of Balb/c 3T3 cells growing in tumor conditioned medium by Lucifer Yellow CH dye-transfer and BrdU incorporation assays. Six to 24 h incubation of Balb/c 3T3 cells in a medium conditioned by WiDr adenocarcinoma cells enhanced the gap junctional communication between the cells by 25-40% as revealed by intercellular transfer of a fluorescent dye Lucifer Yellow CH. Simultaneously the cell proliferation rates were examined and found to be reduced by 23% at 24 h treatment. Since adenocarcinoma cells are known to secrete different growth factor-like polypeptides into their conditioned medium, we suppose that tumors that produce these molecules might alter their host environment through the enhancement of cell-cell communication thereby facilitating the exchange of modulatory factors. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalCancer Letters
Volume151
Issue number1
DOIs
Publication statusPublished - Apr 3 2000

Keywords

  • Balb/c 3T3 cells
  • Cell proliferation
  • Intercellular communication
  • Tumor-conditioned medium
  • WiDr cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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