Effect of trehalose in low concentration on the binding and transport of porphyrins in liposome-human serum albumin system

Irén Bárdos-Nagy, Rita Galántai, J. Fidy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The influence of trehalose on the interaction of liposomes with porphyrins and with human serum albumin (HSA) was studied. Small unilamellar liposomes were prepared from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) and from DMPC/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG) 19:1 w/w% and incorporated with mesoporphyrin IX (MP) or magnesium mesoporphyrin (MgMP). The fluorescence intensity and anisotropy of porphyrins were measured within the temperature range of 15-33°C, in the presence and in the absence of 3×10-2 M trehalose, to study the location of the porphyrins inside the liposomes and their partition between the liposomes and HSA. Based on the presented data and our earlier results (I. Bárdos-Nagy, R. Galántai, A.D. Kaposi, J. Fidy, Int. J. Pharm. 175 (1998) 255-267) we conclude that trehalose - even at this relatively low concentration - interacts with the head groups of the liposomes and that the presence of DMPG enhances the effect. This effect seems to hinder the binding of HSA to the liposome surface and influences the location of MgMP within the liposomes. In the case of MP, the porphyrin partition between the liposomes and HSA was affected by trehalose, while for MgMP, trehalose changed the structural conditions of porphyrin binding to the liposomes. The amount of trehalose used did not have a general trend to modify the association constants of porphyrin derivatives either to liposomes or to HSA.

Original languageEnglish
Pages (from-to)125-134
Number of pages10
JournalBBA - Biomembranes
Volume1512
Issue number1
DOIs
Publication statusPublished - May 2 2001

Fingerprint

Trehalose
Porphyrins
Serum Albumin
Liposomes
Phosphatidylglycerols
Phosphatidylcholines
Unilamellar Liposomes
Fluorescence Polarization
Anisotropy
Fluorescence
Head
Derivatives
Temperature

Keywords

  • Fluorescence
  • Human serum albumin
  • Liposome
  • Magnesium mesoporphyrin
  • Mesoporphyrin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Effect of trehalose in low concentration on the binding and transport of porphyrins in liposome-human serum albumin system. / Bárdos-Nagy, Irén; Galántai, Rita; Fidy, J.

In: BBA - Biomembranes, Vol. 1512, No. 1, 02.05.2001, p. 125-134.

Research output: Contribution to journalArticle

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