Effect of transforming growth factor-β1 on microglial MHC-class II expression

Tamas Pazmany, Janos P. Kosa, Thomas B. Tomasi, Laszlo Mechtler, Andrea Turoczi, Attila Lehotzky

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In the present report, the effects of IFN-γ and transforming growth factor β1 (TGF-β1) on major histocompatibility complex class II (MHC-II) gene expression in isolated mouse brain microglial cells, in the MH-S macrophage cell line and in the primary mouse macrophage cultures were examined. IFN-γ is a potent inducer of MHC-II gene and this induction was further elevated in microglia by TGF-β1, while TGF-β1 inhibited IFN-γ induction in macrophages. The enhancing effect of TGF-β1 was also detected in microglia at the protein level. Transient transfection of microglia with 5' deletional mutants of the MHC-II IA(α) promoter linked to the chloramphenicol acetyltransferase reporter gene demonstrated that TGF-β1 acts at the transcriptional level to enhance the MHC-II expression induced by IFN-γ. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)122-130
Number of pages9
JournalJournal of Neuroimmunology
Volume103
Issue number2
DOIs
Publication statusPublished - Jan 3 2000

Keywords

  • MHC-II
  • Microglia
  • Neurodegenerative diseases
  • TGF-β

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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