Effect of tobamovirus infection on thermoluminescence characteristics of chloroplasts from infected plants

Jaber Rahoutei, Matilde Barón, Isabel García-Luque, Magdolna Droppa, András Neményi, Gábor Horváth

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Changes of thermoluminescence characteristics as well as the O2-evolving capacity was analysed in chloroplasts isolated from Nicotiana benthamiana infected with pepper and paprika mild mottle viruses and their chimeric hybrids. The electron transport activity in thylakoids of virus-infected plants was inhibited and could be restored by adding DPC or Ca2+ which indicated that the virus infection altered the oxygen-evolving complex. In thermoluminescence characteristics of plants infected with either viruses, the first well defined response was a shift in the peak position of the B band from 20 °C to 35 °C corresponding to S3(S2)Q(B)- and S2Q(B)- charge recombinations, respectively, which showed an inhibition in the formation of higher S states in the water splitting system. Simultaneously, a new band appeared around 70 °C due to chemiluminescence of lipid peroxidation. Further progress of the viral infection dramatically decreased the intensity of bands originated from charge recombinations with a concomitant increase of the band at 70 °C indicating the general oxidative breakdown of injured thylakoids.

Original languageEnglish
Pages (from-to)634-639
Number of pages6
JournalZeitschrift fur naturforschung - section c journal of biosciences
Volume54
Issue number9-10
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Biotic Stress
  • Photosynthetic Electron Transport
  • Photosystem II
  • Thermoluminescence
  • Virus Infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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